CAJ | 학술논문

目的探讨自细胞介素-24(又称黑色素瘤分化相关基因-7,IL-24/mda-7)对白血病细胞系K562的周期阻滞作用机制.方法利用基因芯片技术初步分析转染IL-24/mda-7与转染空载体的K562细胞之间基因表达差异,并以实时定量PCR验证;以Western blotting方法检测pRb的磷酸化水平.结果转染IL-24/mda-7可使K562细胞的细胞周期相关基因p21~(WAF-1)、BCCIP上调,cdk6、Smurf2下调,定量PCR证实了上述表达变化;IL-24/mda-7转染还可明显降低K562细胞pRb磷酸化水平.结论 IL-24/mda-7可能通过调节细胞周期相关蛋白,即上调p21~(WAF-1)、BCCIP,下调cdk6、Smurf2,使K562阻滞于G_0/G_1期,从而抑制细胞生长.
목적 탐토자세포개소-24(우칭흑색소류분화상관기인-7,IL-24/mda-7)대백혈병세포계K562적주기조체작용궤제.방법 이용기인심편기술초보분석전염IL-24/mda-7여전염공재체적K562세포지간기인표체차이,병이실시정량PCR험증;이Western blotting방법검측pRb적린산화수평.결과 전염IL-24/mda-7가사K562세포적세포주기상관기인p21~(WAF-1)、BCCIP상조,cdk6、Smurf2하조,정량PCR증실료상술표체변화;IL-24/mda-7전염환가명현강저K562세포pRb린산화수평.결론 IL-24/mda-7가능통과조절세포주기상관단백,즉상조p21~(WAF-1)、BCCIP,하조cdk6、Smurf2,사K562조체우G_0/G_1기,종이억제세포생장.
Objective To explore the mechanism of the cell-cycle arrest induced by human melanoma differentiation associated gene-7 (mda-7/IL-24) in chronic myelocytic leukemia cell line K562. Methods Microarray analysis was performed to determine the genes that were differentially regulated by mda-7/IL-24 in K562 cells, and was validated by realtime PCR. The phosphorylated pRb were detected by Western blotting analysis. Results A microarray analysis showed that G_0/G_1 phase-associated genes p21~(WAF-1) and BCCIP were up-egulated, while cdk6 and Smurf2 were down-regulated. The directional change in the expression of the four genes was successfully validated with real-time quantitative RT-PCR. pRb Set~(795) phosphorylation was observed with no modification of the pRb protein level. Conclusion These results suggest that IL-24/mda-7 may inhibit K562 proliferation and induce G_0/G_1 cell cycle angst by up-regulating p21~(WAF-1) and BCCIP, down-regulating cdk6 and Smurf2.