CAJ | 학술논문

目的探讨亲代大鼠配对前经历吗啡成瘾及戒断对子代焦虑样行为产生的机制.方法选用8周Sprague-Dawley大鼠(雌雄各半)建立吗啡依赖模型,自然戒断21d后配对分组合笼,同时建立生理盐水对照组.子代鼠8周时,雌雄各5只取脑通过Golgi-Cox 染色观察子鼠海马CA1区神经元树突形态情况；另外,子代鼠雌雄再各取5只,深度麻醉后取海马组织作全基因组表达谱分析.结果吗啡成瘾组子代海马CA1区神经元基树突分枝总长度和基树突分支个数低于对照组子代(P＜0.05)；吗啡成瘾组子代与对照组子代相比,雄性子代鼠共同表达2倍上调的有663个基因,共同表达2倍下调的有449个基因,雌性子代鼠共同表达2倍上调的有350个基因,共同表达2倍下调的有188个基因；其中与情绪行为调节相关的基因有:5-HT2c受体上调7倍,Igf-2上调7.1倍,reelin表达下调3.3倍.结论亲代经历吗啡成瘾戒断导致子代海马CA1区神经元树突发育不良,5-HT2c、Igf-2、reelin等基因表达异常,这可能是子代焦虑样行为产生的原因.
목적 탐토친대대서배대전경력마배성은급계단대자대초필양행위산생적궤제.방법 선용8주Sprague-Dawley대서(자웅각반)건립마배의뢰모형,자연계단21d후배대분조합롱,동시건립생리염수대조조.자대서8주시,자웅각5지취뇌통과Golgi-Cox 염색관찰자서해마CA1구신경원수돌형태정황；령외,자대서자웅재각취5지,심도마취후취해마조직작전기인조표체보분석.결과 마배성은조자대해마CA1구신경원기수돌분지총장도화기수돌분지개수저우대조조자대(P＜0.05)；마배성은조자대여대조조자대상비,웅성자대서공동표체2배상조적유663개기인,공동표체2배하조적유449개기인,자성자대서공동표체2배상조적유350개기인,공동표체2배하조적유188개기인；기중여정서행위조절상관적기인유:5-HT2c수체상조7배,Igf-2상조7.1배,reelin표체하조3.3배.결론 친대경력마배성은계단도치자대해마CA1구신경원수돌발육불량,5-HT2c、Igf-2、reelin등기인표체이상,저가능시자대초필양행위산생적원인.
Objective To explore the possible mechanism of adult offspring rats'anxiety-like behavior induced by parents experienced morphine addiction and withdrawal.Methods Establishing the model of Sprague-Dawley rats morphine addiction,Male and female rats were mated after morphine withdrawal 21 days.Meaning-while,saline control group was established in the same method.5 female and 5 male offspring's brains were obtained to observe the neuronal morphology of hippocampal CA1 through Golgi staining when they were 8 weeks old,the same number of female and male's hippocampus were derived after deeply anesthetized to perform the whole genome expression profiles analysis.Results The total length and the number of basal dendrites branches on hippocampal CA1 neurons in offspring of morphine groups were significantly decreased compared to the offspring of saline group.Comparison with the offspring of saline group,663 up-regulated genes (ratios ≥2.0) and 499 down-regulated genes ( ratios ≤0.5 ) were detected in the male offspring of morphine groups,and 350 up-regulated genes (ratios ≥2.0) and 188 down-regulated genes (ratios ≤0.5) were done in the female.Furthermore,they included many genes associated with regulation of emotional behavior,such as 5-HT2c receptor up-regulation 7-fold,Igf-2 up-regulation 7.1-fold and reelin down-regulation 3.3-fold were observed.Conclusion Experienced morphine addiction and withdrawal in parents prior to mating leads to dysplasia of dendritic morphology in hippocampal CA1 neurons of adult offspring rats,and 5-HT2c,Igf-2,reelin expressing abnormally,which may be the possible mechanism of anxiety-like behavior in adult offspring rats.