中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2009年
1期
54-57
,共4页
棘球蚴病%疫苗,合成%脾%T淋巴细胞亚群
棘毬蚴病%疫苗,閤成%脾%T淋巴細胞亞群
극구유병%역묘,합성%비%T림파세포아군
Echinococcosis%Vaccines,synthetic%Spleen%T lymphocyte subsets
目的 动态观察细粒棘球绦虫重组BCG-Eg95疫苗免疫小鼠后脾细胞亚群的变化.方法 将120只Balb/c小鼠按体质量随机分为3组:鼻腔内接种组、口服灌胃组、对照组.将疫苗分别采用鼻腔内接种和口服灌胃免疫实验组小鼠,对照组单次接种10μl磷酸盐缓冲液(PBS)于小鼠鼻腔黏膜.在免疫后0、2、4、6、8、10、12、14、16、18周各剖杀4只小鼠,取脾脏,分离脾细胞,流式细胞仪检测脾CD4+、CD8+T淋巴细胞亚群的百分比.结果 不同组的小鼠脾细胞CD4+、CD8+亚群百分比比较差异有统计学意义(F值分别为21.56、22.08,P＜0.05),不同周数的小鼠脾细胞CD4+、CD8+亚群百分比比较差异有统计学意义(F值分别为5.75、6.29.P＜0.01).鼻腔内接种组的脾CD4+、CD8+T细胞亚群分别在免疫后6-18、12周升高,在免疫后10、12周达最高水平,其值分别为0.348±0.013、0.090±0.003.与0周(0.230±0.022、0.069±0.015)比较差异有统计学意义(q值分别为7.32、5.32,P＜0.01或＜0.05);口服灌胃组脾细胞CD4+、CD8+T细胞亚群分别在免疫后6～16、8-18周增加,分别在免疫后10、16周达最高水平,其值分别为0.405±0.006、0.096±0.004,与0周(0.230±0.022、0.069±0.015)比较差异有统计学意义(q值分别为7.53、5.35,P＜0.01或＜0.05).结论 CD4+、CD8+T细胞亚群在细粒棘球绦虫重组BCG-Eg95疫苗诱导的小鼠免疫应答中起重要作用.
目的 動態觀察細粒棘毬縚蟲重組BCG-Eg95疫苗免疫小鼠後脾細胞亞群的變化.方法 將120隻Balb/c小鼠按體質量隨機分為3組:鼻腔內接種組、口服灌胃組、對照組.將疫苗分彆採用鼻腔內接種和口服灌胃免疫實驗組小鼠,對照組單次接種10μl燐痠鹽緩遲液(PBS)于小鼠鼻腔黏膜.在免疫後0、2、4、6、8、10、12、14、16、18週各剖殺4隻小鼠,取脾髒,分離脾細胞,流式細胞儀檢測脾CD4+、CD8+T淋巴細胞亞群的百分比.結果 不同組的小鼠脾細胞CD4+、CD8+亞群百分比比較差異有統計學意義(F值分彆為21.56、22.08,P＜0.05),不同週數的小鼠脾細胞CD4+、CD8+亞群百分比比較差異有統計學意義(F值分彆為5.75、6.29.P＜0.01).鼻腔內接種組的脾CD4+、CD8+T細胞亞群分彆在免疫後6-18、12週升高,在免疫後10、12週達最高水平,其值分彆為0.348±0.013、0.090±0.003.與0週(0.230±0.022、0.069±0.015)比較差異有統計學意義(q值分彆為7.32、5.32,P＜0.01或＜0.05);口服灌胃組脾細胞CD4+、CD8+T細胞亞群分彆在免疫後6～16、8-18週增加,分彆在免疫後10、16週達最高水平,其值分彆為0.405±0.006、0.096±0.004,與0週(0.230±0.022、0.069±0.015)比較差異有統計學意義(q值分彆為7.53、5.35,P＜0.01或＜0.05).結論 CD4+、CD8+T細胞亞群在細粒棘毬縚蟲重組BCG-Eg95疫苗誘導的小鼠免疫應答中起重要作用.
목적 동태관찰세립극구조충중조BCG-Eg95역묘면역소서후비세포아군적변화.방법 장120지Balb/c소서안체질량수궤분위3조:비강내접충조、구복관위조、대조조.장역묘분별채용비강내접충화구복관위면역실험조소서,대조조단차접충10μl린산염완충액(PBS)우소서비강점막.재면역후0、2、4、6、8、10、12、14、16、18주각부살4지소서,취비장,분리비세포,류식세포의검측비CD4+、CD8+T림파세포아군적백분비.결과 불동조적소서비세포CD4+、CD8+아군백분비비교차이유통계학의의(F치분별위21.56、22.08,P＜0.05),불동주수적소서비세포CD4+、CD8+아군백분비비교차이유통계학의의(F치분별위5.75、6.29.P＜0.01).비강내접충조적비CD4+、CD8+T세포아군분별재면역후6-18、12주승고,재면역후10、12주체최고수평,기치분별위0.348±0.013、0.090±0.003.여0주(0.230±0.022、0.069±0.015)비교차이유통계학의의(q치분별위7.32、5.32,P＜0.01혹＜0.05);구복관위조비세포CD4+、CD8+T세포아군분별재면역후6～16、8-18주증가,분별재면역후10、16주체최고수평,기치분별위0.405±0.006、0.096±0.004,여0주(0.230±0.022、0.069±0.015)비교차이유통계학의의(q치분별위7.53、5.35,P＜0.01혹＜0.05).결론 CD4+、CD8+T세포아군재세립극구조충중조BCG-Eg95역묘유도적소서면역응답중기중요작용.
Objective To dynamically observe the changes of subsets of splenocytes in mice by immunization with recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg).Methods Balb/c mice were divided randomly into 3 groups according to their weishts:intranasal group.per os group and PBS control.The mice were vaccinated intranasally or orally by the vaccine respectively in experimental groups.and the control mice were given phosphate buffer saline intranasally.These mice were killed to get spleen on 0,2,4,6,8,10,12, 14,16 and 18 week of immunization,respectively.Splenocytes were separated to measure subsets of CD4+ and CD8+T ceUs by FACsort.Results There were marked differences in ratio of CD4+ and CD8+ subsets among the different groups(F value were 21.56 and 22.08 respectively,P＜0.05).There were very marked differences in ratio of CD4+ and CD8+ subsets in different weeks(F value were 5.75 and 6.29 respectively.P＜0.01).In the intranasal group,CD4+ and CD8+ T subsets increased obviously in 6～18 weeks and 12 weeks,and reached the highest level on 10 and 12 week,espectively.Their values were 0.348±0.013 and 0.090±0.003.respectively.There were marked or very marked differences(q value were 7.32 and 5.32 respectively,P＜0.01 or＜0.05)in comparison with 0 week(0.230±0.022 and 0.069±0.015).In the oral group,CD4+and CD8+ subsets rose reinarkablv on 6-16 weeks and 8-18 weeks,achieved the hishest level on 10 and 16 weeks,respectively.Their vahes were 0.405± 0.006 and 0.096±0.004,respectively.There were marked or very marked difference(q value were 7.53 and 5.35 respectively,P＜0.01 or＜0.05)in comparison with week 0(0.230±0.022 and 0.069±0.015).Conclusion CD4+and CD8+T subsets may play an important role in immune response induced in mice by rBCG-Eg95 vaccine.
