中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2009年
7期
580-582
,共3页
益智仁%脑缺血%记忆障碍
益智仁%腦缺血%記憶障礙
익지인%뇌결혈%기억장애
Alpinia oxyphylla Miq. fruit%Cerebral ischemia%Memory impairment
目的 探讨益智仁水提取物(AOF)对脑缺血大鼠学习记忆能力的改善作用及其作用机制.方法 48只健康成年雄性SD大鼠随机分成4组:假手术组、脑缺血组、AOF Ⅰ组、AOF Ⅱ组,每组12只.除假手术组外,其余各组大鼠均采用双侧颈总动脉反复夹闭再灌注同时腹腔注射硝普钠降压方法建立脑缺血动物模型.避暗实验测定大鼠学习记忆能力,同时测定海马一氧化氮(NO)含量及一氧化氮合酶(NOS)活力.结果 避暗实验中,脑缺血组大鼠的潜伏期[(143.8±65.2)s]显著短于假手术组[(257.2±67.1)s](P<0.01),错误次数[(8.9±4.2)次]显著多于假手术组[(1.7±1.1)次](P<0.01);而AOF Ⅰ、Ⅱ组的潜伏期[(186.5±46.2)s,(193.4±43.7)s]显著长于脑缺血组(P<0.05),错误次数[(6.1±2.9)次,(5.2±2.1)次]显著少于脑缺血组(P<0.05,P<0.01).脑缺血组海马NO含量及NOS活力[(56.53±27.42)nmol/mg prot,(17.23±5.64)nmol/mg prot]显著高于假手术组[(40.02±17.9)nmol/mg prot,(10.46±6.15)nmol/mg prot];而AOF Ⅰ、Ⅱ组海马NO含量及NOS活力[NO含量:Ⅰ组(46.60±20.26)nmol/mg prot,Ⅱ组(42.38±21.23)nmol/mg prot;NOS活力:Ⅰ组(13.98±5.13)nmol/mg prot,Ⅱ组(13.61±5.27)nmol/mg prot]显著低于脑缺血组(P<0.05).结论 AOF可显著改善脑缺血所致大鼠记忆障碍,其机制可能与降低海马NO含量及NOS活力有关.
目的 探討益智仁水提取物(AOF)對腦缺血大鼠學習記憶能力的改善作用及其作用機製.方法 48隻健康成年雄性SD大鼠隨機分成4組:假手術組、腦缺血組、AOF Ⅰ組、AOF Ⅱ組,每組12隻.除假手術組外,其餘各組大鼠均採用雙側頸總動脈反複夾閉再灌註同時腹腔註射硝普鈉降壓方法建立腦缺血動物模型.避暗實驗測定大鼠學習記憶能力,同時測定海馬一氧化氮(NO)含量及一氧化氮閤酶(NOS)活力.結果 避暗實驗中,腦缺血組大鼠的潛伏期[(143.8±65.2)s]顯著短于假手術組[(257.2±67.1)s](P<0.01),錯誤次數[(8.9±4.2)次]顯著多于假手術組[(1.7±1.1)次](P<0.01);而AOF Ⅰ、Ⅱ組的潛伏期[(186.5±46.2)s,(193.4±43.7)s]顯著長于腦缺血組(P<0.05),錯誤次數[(6.1±2.9)次,(5.2±2.1)次]顯著少于腦缺血組(P<0.05,P<0.01).腦缺血組海馬NO含量及NOS活力[(56.53±27.42)nmol/mg prot,(17.23±5.64)nmol/mg prot]顯著高于假手術組[(40.02±17.9)nmol/mg prot,(10.46±6.15)nmol/mg prot];而AOF Ⅰ、Ⅱ組海馬NO含量及NOS活力[NO含量:Ⅰ組(46.60±20.26)nmol/mg prot,Ⅱ組(42.38±21.23)nmol/mg prot;NOS活力:Ⅰ組(13.98±5.13)nmol/mg prot,Ⅱ組(13.61±5.27)nmol/mg prot]顯著低于腦缺血組(P<0.05).結論 AOF可顯著改善腦缺血所緻大鼠記憶障礙,其機製可能與降低海馬NO含量及NOS活力有關.
목적 탐토익지인수제취물(AOF)대뇌결혈대서학습기억능력적개선작용급기작용궤제.방법 48지건강성년웅성SD대서수궤분성4조:가수술조、뇌결혈조、AOF Ⅰ조、AOF Ⅱ조,매조12지.제가수술조외,기여각조대서균채용쌍측경총동맥반복협폐재관주동시복강주사초보납강압방법건립뇌결혈동물모형.피암실험측정대서학습기억능력,동시측정해마일양화담(NO)함량급일양화담합매(NOS)활력.결과 피암실험중,뇌결혈조대서적잠복기[(143.8±65.2)s]현저단우가수술조[(257.2±67.1)s](P<0.01),착오차수[(8.9±4.2)차]현저다우가수술조[(1.7±1.1)차](P<0.01);이AOF Ⅰ、Ⅱ조적잠복기[(186.5±46.2)s,(193.4±43.7)s]현저장우뇌결혈조(P<0.05),착오차수[(6.1±2.9)차,(5.2±2.1)차]현저소우뇌결혈조(P<0.05,P<0.01).뇌결혈조해마NO함량급NOS활력[(56.53±27.42)nmol/mg prot,(17.23±5.64)nmol/mg prot]현저고우가수술조[(40.02±17.9)nmol/mg prot,(10.46±6.15)nmol/mg prot];이AOF Ⅰ、Ⅱ조해마NO함량급NOS활력[NO함량:Ⅰ조(46.60±20.26)nmol/mg prot,Ⅱ조(42.38±21.23)nmol/mg prot;NOS활력:Ⅰ조(13.98±5.13)nmol/mg prot,Ⅱ조(13.61±5.27)nmol/mg prot]현저저우뇌결혈조(P<0.05).결론 AOF가현저개선뇌결혈소치대서기억장애,기궤제가능여강저해마NO함량급NOS활력유관.
Objective This report investigate the intervention effect of the water extract of Alpinia oxyphylla Miq. fruit (AOF)on memory impairment and the mechanism in cerebral ischemia rats. Methods 48 Rats were randomly divided into sham-operated group(n=12), ischemia group(n=12), AOF group Ⅰ( n= 12)and AOF group Ⅱ (n= 12). The model of transient cerebral ischemic/reperfusion was made by bilateral occlusion of common carotid arteries in rats and combination with reducing blood pressure with an abdominal injection of so-dium nitroprusside. Learning-memory ability was observed by step through test. The content of NO and the activi-ties of NOS were measured in hippocampus. Results In step through test,the latency in ischemia group[(143.8±65.2)s]significantly decreased, the number of errors (8.9±4.2 ) significantly increased compared with sham-operated group [latency: (257.2±67.1 ) s; number of errors: (1.7±1.1 ), P<0.01 ]. The latency in AOF group Ⅰ and AOF group Ⅱ[(186.5±46.2) s, (193.4±43.7 ) s ] significantly increased, the number of errors (6.1±2.9,5.2±2.1 ) significantly decreased compared with ischemia group(P<0.05, P<0.01). In hippocampus, the content of NO and the activities of NOS in ischemia group [(56.53±27.42) nmol/mg prot, (17.23±5.64) nmol/mg prot] significantly increased compared with sham-operated group[ (40.02±17.9 ) nmol/mg prot, ( 10.46±6.15)nmol/mg prot], and in AOF group Ⅰ and AOF group Ⅱ [content of NO:group Ⅰ (46.60 ±20. 26)nmol/mg prot,group Ⅱ (42.38±21.23) nmol/mg prot ;activities of NOS:group Ⅰ (13.98±5.13 ) nmol/mg pint,group Ⅱ(13.61±5.27) nmol/mg prot] significantly decreased compared with ischemia group(P<0.05). Conclu-sion AOF could significantly ameliorate the memory impairment in cerebral ischemic rats. Its effects may be in-volved in the decrease of content of NO and activities of NOS in hippocampus.
