CAJ | 학술논문

目的探讨转化生长因子β1基因与内皮素转换酶-1b基因多态性和颈动脉粥样硬化易感性的关联情况。方法选择西安交通大学医学院第一附属医院神经内科自2006年1月至2010年12月收治的231例脑梗死住院患者为患者组，同期社区健康自愿参与研究者231例为对照组，运用PCR及单核苷酸多态性技术分析2组成员转化生长网子β1基因与内皮素转换酶-1b基因多态性分型，比较2组间基因和基因型差异。结果患者组和对照组转化生长因子β1基因的rs51695112位点基因和基因型差异有统计学意义(x2=6.120、6.890，P=0.045、0.033)，G等位基因和G/G基因型为颈动脉粥样硬化易感性的高危因素(OR=1.320、1.410)；患者组和对照组内皮素转换酶-1b基因rs32570233位点基因和基因型差异有统计学意义(x2=6.320、7.180，P=0.041、0.022），A等位基因和A/A基因型为颈动脉粥样硬化易感性的高危因素(OR=1.470、1.530)。结论转化生长因子β1基因、内皮素转换酶-1b基因多态性与颈动脉粥样硬化相关联。
목적탐토전화생장인자β1기인여내피소전환매-1b기인다태성화경동맥죽양경화역감성적관련정황。 방법선택서안교통대학의학원제일부속의원신경내과자2006년1월지2010년12월수치적231례뇌경사주원환자위환자조，동기사구건강자원삼여연구자231례위대조조，운용PCR급단핵감산다태성기술분석2조성원전화생장망자β1기인여내피소전환매-1b기인다태성분형，비교2조간기인화기인형차이。 결과환자조화대조조전화생장인자β1기인적rs51695112위점기인화기인형차이유통계학의의(x2=6.120、6.890，P=0.045、0.033)，G등위기인화G/G기인형위경동맥죽양경화역감성적고위인소(OR=1.320、1.410)；환자조화대조조내피소전환매-1b기인rs32570233위점기인화기인형차이유통계학의의(x2=6.320、7.180，P=0.041、0.022），A등위기인화A/A기인형위경동맥죽양경화역감성적고위인소(OR=1.470、1.530)。 결론전화생장인자β1기인、내피소전환매-1b기인다태성여경동맥죽양경화상관련。
Objective To study the relationship between polymorphisms of transforming growth factor β1 gene and endothelin converting enzyme-1b gene and susceptibility of carotid atherosclerosis. Methods Two hundred and thirty-one patients with carotid atherosclerosis, admitted to and received treatment in our hospital from January 2006 to December 2010, and 231 healthy volunteers were chosen in our study. The polymorphisms of transforming growth factor β1 gene and endothelin converting enzyme-lb gene in these 2 groups were detected with PCR and single nucleotide polymorphism; the differences of these gene and genotype between the 2 groups were compared by statistical analysis.Results The gene and genotype of transforming growth factor β1 gene at rs51695112 locus were significantly different between patient group and control group (x2=6.120, 6.890;P=0.045, 0.033, respectively); the G allele and G/G genotype were risk factors of carotid atherosclerosis susceptibility (OR=1.320, 1.410, respectively). The gene and genotype of endothelin converting enzyme-1b gene at rs32570233 locus were significantly different between the patient group and the control group (x2=6.320, 7.180; P=0.041, 0.022); the A allele and A/A genotype were the risk factors of carotid atherosclerosis susceptibility (OR=1.470, 1.530, respectively). Conclusion There is an association between polymorphisms of transforming growth factor β 1 gene and endothelin converting enzyme-1b gene and carotid atherosclerosis.