CAJ | 학술논문

目的观察地塞米松对兔椎间盘髓核细胞增殖的影响,以及对髓核细胞分泌聚集蛋白聚糖(Aggrecan)的影响.方法无菌条件下取兔椎间盘,常规分离消化髓核细胞进行培养;传代培养第2代髓核细胞7 d,随机分为两组,实验组给于1～10000 nmol/L地塞米松进行培养,对照组不给于地塞米松,分别培养不同的时间段.采用噻唑蓝(MTT)比色法检测地塞米松对兔髓核细胞增殖的影响;采用逆转录-聚合酶链反应(RT-PCR)法检测地塞米松对兔髓核细胞内Aggrecan mRNA表达的影响.结果 MTT检测结果表明地塞米松对兔髓核细胞增殖有促进作用,最佳作用浓度为100nmol/L,最佳作用时间为48 h;RT-PCR检测结果表明经地塞米松处理的髓核细胞其Aggrecan表达明显较对照组高,是对照组的2.04倍(0.92/0.45),差异有统计学意义(P<0.05).结论地塞米松可促进兔椎间盘髓核细胞的增殖,并可使兔髓核细胞内Aggrecan表达增高.
목적 관찰지새미송대토추간반수핵세포증식적영향,이급대수핵세포분비취집단백취당(Aggrecan)적영향.방법 무균조건하취토추간반,상규분리소화수핵세포진행배양;전대배양제2대수핵세포7 d,수궤분위량조,실험조급우1～10000 nmol/L지새미송진행배양,대조조불급우지새미송,분별배양불동적시간단.채용새서람(MTT)비색법검측지새미송대토수핵세포증식적영향;채용역전록-취합매련반응(RT-PCR)법검측지새미송대토수핵세포내Aggrecan mRNA표체적영향.결과 MTT검측결과표명지새미송대토수핵세포증식유촉진작용,최가작용농도위100nmol/L,최가작용시간위48 h;RT-PCR검측결과표명경지새미송처리적수핵세포기Aggrecan표체명현교대조조고,시대조조적2.04배(0.92/0.45),차이유통계학의의(P<0.05).결론 지새미송가촉진토추간반수핵세포적증식,병가사토수핵세포내Aggrecan표체증고.
Objective To study the proliferative effects of dexamethasone on rabbit nucleus pulpo-sus cell, and the expression of Aggreean in nucleus pulposus cells. Methods The intervertebral disk was extracted by sterile process, and after digestion of nucleus pulposus the cells were cultured in DMEM. 1-10 000 nmol/L of dexamethasone was added into cultured nucleus pulposus colls. The proliferative effects of dexamethasone on nucleus pulposus cells were measured by MTT assay, and the expression of Aggrecan mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results MTT revealed that dexamethasone had the proliferative effects on cultured nucleus pulposus cells, and the optimal treated time was 48 h, the optimam concentration was 100 nmol/L. The RT-PCR results showed that dexamethasone could up-regulate the expression of Aggrecan mRNA as compared with control group. Conclusion Dexam-ethasone could promote nucleus pulposus cell proliferation and up-regulate the expression of Aggrecan.