中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
5期
662-664
,共3页
周晓妮%杨欢%温会燕%周惠琴%冯萍%朱雪明
週曉妮%楊歡%溫會燕%週惠琴%馮萍%硃雪明
주효니%양환%온회연%주혜금%풍평%주설명
Toll样受体%乳腺癌%转移
Toll樣受體%乳腺癌%轉移
Toll양수체%유선암%전이
TLRs%Breast carcinoma%Invasion
目的 观察人高低转移能力乳腺癌细胞株中Toll样受体(TLRs)的表达,探讨差异表达及意义.方法 选取高转移能力的人乳腺癌细胞株MDA-MB231和低转移能力的人乳腺癌细胞株MCF-7,采用逆转录-聚合酶链反应(RT-PCR)、实时荧光定量PCR、流式细胞技术和细胞免疫荧光技术检测TLR1～TLR10 mRNA水平和蛋白水平的表达,观察两株细胞TLRs的表达及表达差异.结果 人乳腺癌细胞株MDA-MB-231和MCF-7 mRNA水平和蛋白水平都广泛表达TLRs包括TLR1、TLR2、TLR3、TLR4、TLR5、TLR6、TLR7、TLR8、TLR9、TLR10.两细胞株mRNA水平表达差异最明显的是TLR4、TLR6、TLR8和TLR9,MDA-MB-231中TLR4、TLR6、TLR8和TLR9的表达分别是MCF-7的(39.4±3.2)倍、(10.8±2.4)倍、(5.8±2.6)倍和(3.2±2.5)倍(P＜0.01);两细胞株蛋白表达差异最大的是TLR4、TLR6、TLR7和TLR5,MDA-MB-231中TLR4、TLR6、TLR7和TLR5的表达分别是MCF-7的(6.4±1.6)倍、(4.1±0.9)倍、(3.7±0.8)倍和(2.8±0.4)倍(P＜0.01).结论 TLRs在人高低转移能力乳腺癌细胞株广泛表达且表达水平不一;TLR4是表达差异最明显的受体;差异表达的,TLRs可能与乳腺癌细胞转移能力相关.
目的 觀察人高低轉移能力乳腺癌細胞株中Toll樣受體(TLRs)的錶達,探討差異錶達及意義.方法 選取高轉移能力的人乳腺癌細胞株MDA-MB231和低轉移能力的人乳腺癌細胞株MCF-7,採用逆轉錄-聚閤酶鏈反應(RT-PCR)、實時熒光定量PCR、流式細胞技術和細胞免疫熒光技術檢測TLR1～TLR10 mRNA水平和蛋白水平的錶達,觀察兩株細胞TLRs的錶達及錶達差異.結果 人乳腺癌細胞株MDA-MB-231和MCF-7 mRNA水平和蛋白水平都廣汎錶達TLRs包括TLR1、TLR2、TLR3、TLR4、TLR5、TLR6、TLR7、TLR8、TLR9、TLR10.兩細胞株mRNA水平錶達差異最明顯的是TLR4、TLR6、TLR8和TLR9,MDA-MB-231中TLR4、TLR6、TLR8和TLR9的錶達分彆是MCF-7的(39.4±3.2)倍、(10.8±2.4)倍、(5.8±2.6)倍和(3.2±2.5)倍(P＜0.01);兩細胞株蛋白錶達差異最大的是TLR4、TLR6、TLR7和TLR5,MDA-MB-231中TLR4、TLR6、TLR7和TLR5的錶達分彆是MCF-7的(6.4±1.6)倍、(4.1±0.9)倍、(3.7±0.8)倍和(2.8±0.4)倍(P＜0.01).結論 TLRs在人高低轉移能力乳腺癌細胞株廣汎錶達且錶達水平不一;TLR4是錶達差異最明顯的受體;差異錶達的,TLRs可能與乳腺癌細胞轉移能力相關.
목적 관찰인고저전이능력유선암세포주중Toll양수체(TLRs)적표체,탐토차이표체급의의.방법 선취고전이능력적인유선암세포주MDA-MB231화저전이능력적인유선암세포주MCF-7,채용역전록-취합매련반응(RT-PCR)、실시형광정량PCR、류식세포기술화세포면역형광기술검측TLR1～TLR10 mRNA수평화단백수평적표체,관찰량주세포TLRs적표체급표체차이.결과 인유선암세포주MDA-MB-231화MCF-7 mRNA수평화단백수평도엄범표체TLRs포괄TLR1、TLR2、TLR3、TLR4、TLR5、TLR6、TLR7、TLR8、TLR9、TLR10.량세포주mRNA수평표체차이최명현적시TLR4、TLR6、TLR8화TLR9,MDA-MB-231중TLR4、TLR6、TLR8화TLR9적표체분별시MCF-7적(39.4±3.2)배、(10.8±2.4)배、(5.8±2.6)배화(3.2±2.5)배(P＜0.01);량세포주단백표체차이최대적시TLR4、TLR6、TLR7화TLR5,MDA-MB-231중TLR4、TLR6、TLR7화TLR5적표체분별시MCF-7적(6.4±1.6)배、(4.1±0.9)배、(3.7±0.8)배화(2.8±0.4)배(P＜0.01).결론 TLRs재인고저전이능력유선암세포주엄범표체차표체수평불일;TLR4시표체차이최명현적수체;차이표체적,TLRs가능여유선암세포전이능력상관.
Objective To study the expression and significances of Toll like receptors (TLRs) in human high invasive breast cancer cells MDA-MB-231 and poor invasive breast cancer cells MCF-7. Methods TLRs mRNA expression levels were detected in breast cancer cell lines MDA-MB-231 and MCF-7 by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. TLRs protein expression levels were detected by flow cytometry (FCM) and immunofluorescence methods. Results Human breast cancer cell lines MDA-MB-231 and MCF-7 expressed TLRs including TLR1-TLR10 at both mRNA and protein levels. The mRNA expression of TLR4, TLR6, TLR8 and TLR9 in MDA-MB-231 cells was obviously higher than that in MCF-7 cells respectively, and the expression in MDA-MB-231 cells was (39.4 ±3. 2),(10. 8 ± 2. 4), (5. 8 ± 2. 6 ) and (3. 2 ± 2. 5) times as much as that in MCF-7 cells respectively ( P ＜0. 01). Protein expression of TLR4, TLR6, TLR7 and TLR5 in MDA-MB-231 cells was significantly higher than that in MCF-7 cells respectively, and the expression in MDA-MB-231 cells was (6.4 ± 1.6),(4. 1 ± 0. 9), (3. 7 ± 0. 8 ) and (2. 8 ± 0.4) times as much as that in MCF-7 cells respectively ( P ＜0. 01). Conclusion TLR1-TLR10 was expressed widely and differentially in human breast cancer cell lines MDA-MB-231 and MCF-7. TLR4 mRNA and protein levels were the highest out of TLR1-TLR10.The differential expressions of TLRs may be related to the invasion capacity of human breast cancer cells.TLR4 may provide basement for breast cancer target gene therapy.
