CAJ | 학술논문

目的探讨IL-23在缓解期类固醇激素依赖型溃疡性结肠炎患者结肠组织中表达的病理意义.方法采用Western blot分析及免疫组化SABC法检测15例缓解期激素依赖型溃疡性结肠炎患者炎症修复区结肠组织IL-23的蛋白表达情况,予统计学软件统计分析,并以30例缓解期的一般溃疡性结肠炎患者(15例SASP维持治疗,15例强的松维持治疗)炎症修复区结肠组织及10例正常结肠黏膜组织为对照组.结果与正常对照组比较,SASP维持治疗及强的松维持治疗缓解期一般溃疡性结肠炎患者炎症修复区结肠组织IL-23的蛋白表达均轻度升高(P>0.05),而缓解期激素依赖型溃疡性结肠炎患者炎症修复区结肠组织IL-23的蛋白表达显著高于一般溃疡性结肠炎组(P<0.01).结论 IL-23的过度表达可能在溃疡性结肠炎类固醇激素依赖发病机制中起关键作用.
목적 탐토IL-23재완해기류고순격소의뢰형궤양성결장염환자결장조직중표체적병리의의.방법 채용Western blot분석급면역조화SABC법검측15례완해기격소의뢰형궤양성결장염환자염증수복구결장조직IL-23적단백표체정황,여통계학연건통계분석,병이30례완해기적일반궤양성결장염환자(15례SASP유지치료,15례강적송유지치료)염증수복구결장조직급10례정상결장점막조직위대조조.결과 여정상대조조비교,SASP유지치료급강적송유지치료완해기일반궤양성결장염환자염증수복구결장조직IL-23적단백표체균경도승고(P>0.05),이완해기격소의뢰형궤양성결장염환자염증수복구결장조직IL-23적단백표체현저고우일반궤양성결장염조(P<0.01).결론 IL-23적과도표체가능재궤양성결장염류고순격소의뢰발병궤제중기관건작용.
Objective To investigate the pathological significance of expression of IL-23 in colon tissues of patients with steroid-dependent uncreative colitis at remission.Methods Expression of IL-23 was measured by means of Western Blot and immunohistochemistry SABC in inflammation repairing areas from 15 patients with steroid-dependent ulcerative colitis at remission, 30 patients with common ulcerative colitis at remission (15 treated with SASP and 15 with prednisone) and 10 normal colon tissues.The results were analyzed by SPSS 16.0.Results Compared with normal control, expression of IL-23 in patients of SASP maintenance therapy and prednisone with common ulcerative colitis were slightly increased ( P > 0.05), which was significantly lower that of steroid-dependent specimens ( P < 0.01).Conclusion Over-expression of IL-23 may plas a key role in the pathogenesis of steroid-dependent ulcerative colitis.