CAJ | 학술논문

目的探讨聚乙二醇(PEG)包埋小鼠胰岛细胞对同种小鼠胰岛移植物存活的影响,以及其与抗排斥药雷帕霉素共同应用对胰岛移植后抗排斥治疗的影响.方法选取C57BL/16雄性小鼠皮下预血管化糖尿病模型,行BALB/c小鼠胰岛移植.随机分为6组:A组:正常胰岛移植组.B组:PEG包埋胰岛移植组.C组:正常胰岛移植+雷帕霉素1.5 mg·kg~(-1)·d~(-1)治疗组.D组:PEG包埋胰岛移植+雷帕霉素1.5 mg·kg~(-1)·d~(-1)治疗组.E组:正常胰岛移植+雷帕霉素3 mg·kg~(-1)·d~(-1)治疗组.F组:PEG包埋胰岛移植+雷帕霉素3 mg·kg~(-1)·d~(-1)治疗组.观察小鼠血糖变化,病理检测移植物存活情况.结果 B组胰岛存活时间(25.5±5.9)d较A组(14.7±2.6)d明显延长(P<0.01),C组胰岛存活时间(35.0±3.1)d,D、E、F组胰岛于移植后6周均存活.HE染色下B组较A组炎症反应轻,胰岛结构完整.免疫组化染色显示B组有散在染色增强细胞团而A组未见.结论 PEG包埋胰岛细胞可显著延长移植物的存活时间,并能减少免疫抑制剂雷帕霉素的用量.
목적 탐토취을이순(PEG)포매소서이도세포대동충소서이도이식물존활적영향,이급기여항배척약뢰파매소공동응용대이도이식후항배척치료적영향.방법 선취C57BL/16웅성소서피하예혈관화당뇨병모형,행BALB/c소서이도이식.수궤분위6조:A조:정상이도이식조.B조:PEG포매이도이식조.C조:정상이도이식+뢰파매소1.5 mg·kg~(-1)·d~(-1)치료조.D조:PEG포매이도이식+뢰파매소1.5 mg·kg~(-1)·d~(-1)치료조.E조:정상이도이식+뢰파매소3 mg·kg~(-1)·d~(-1)치료조.F조:PEG포매이도이식+뢰파매소3 mg·kg~(-1)·d~(-1)치료조.관찰소서혈당변화,병리검측이식물존활정황.결과 B조이도존활시간(25.5±5.9)d교A조(14.7±2.6)d명현연장(P<0.01),C조이도존활시간(35.0±3.1)d,D、E、F조이도우이식후6주균존활.HE염색하B조교A조염증반응경,이도결구완정.면역조화염색현시B조유산재염색증강세포단이A조미견.결론 PEG포매이도세포가현저연장이식물적존활시간,병능감소면역억제제뢰파매소적용량.
Objective To investigate the impact of polyethylene glycols( PEG) upon islet survival in homogeneous murine islet transplantation and understand the impact of PEGylation in combination with rapamycin upon anti-rejection therapy in homogeneous mice islet transplantation. Methods The subcutaneously pre-vascularized STZ-induced diabetic mice treated with transplanted islets of BALB/c mice were randomly divided into 6 groups: Group A with normal mice islets; Group B with PEG-packed islets; Group C with normal mice islets and 1. 5 mg · kg~(-1)· d~(-1) rapamycin; Group D with PEG-packed islets and 1. 5 mg · kg~(-1) · d~(-1)rapamycin; Group E with normal mice islets and 3 mg · kg~(-1) · d~(-1) rapamycin; Group F with PEG-packed islets and 3 mg · kg~(-1) · d~(-1) rapamycin. The post-transplantation blood glucose was monitored. Transplanted islets were analyzed by H&E and insulin immunostain. Results The survival time in group B was significantly prolonged as compared with group A (P<0. 01). The survival time in group C were (35. 0 ±3. 1 )d and groups D, E, F had survival of up to 6 weeks. Transplantation sites of group A were observed with a more abundant infiltration of immune cells than group B. And the unmodified islets in group A were completely destroyed after transplantation. Insulin-positive islet cells were not detected at the entire transplantation site in group A while the presence was found at the transplantation site in group B. Conclusion PEG-packed islets can significantly improve the survival time of transplanted islets. When combined with rapamycin, it can reduce the dose of rapamycin.