中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2011年
3期
200-205
,共6页
肝炎,乙型%肝炎表面抗原,乙型%抗原抗体反应%免疫测定%儿童,学龄前
肝炎,乙型%肝炎錶麵抗原,乙型%抗原抗體反應%免疫測定%兒童,學齡前
간염,을형%간염표면항원,을형%항원항체반응%면역측정%인동,학령전
Hepatitis B%Hepatitis B surface antigens%Antigen-antibody reactions%Immunoassay%Child,preschool
目的 调查和分析浙江地区婴幼儿和学龄前儿童乙型肝炎(简称乙肝)病毒血清学模式及其与年龄、性别相关的分布特征,为当地儿童乙肝的预防与控制提供依据.方法 采用电化学发光技术在Roche e601免疫分析仪上检测乙肝病毒血清学标志物.应用实验室信息管理系统(LIS)将2006年7月至2010年3月期间所有乙肝病毒血清学检测结果导出为EXCEL数据文件,通过排除重复检测者、年龄＞7周岁和住址非浙江地区者,最终筛选出24 892名研究对象,男15 810名,女9082名.采用SPSS 13.0和EXCEL 2003对乙肝病毒血清学检测结果进行统计分析.结果 乙肝疫苗平均覆盖率在浙江地区15 413名男孩和8 840名女孩中分别为99.16%(15 413/15 543)和99.17%(8 840/8 914).所有24 892名7岁内儿童中检出乙肝病毒血清学模式17种,最主要模式为"单antiHBs(+)",占65.997%(16 179/24 892);其次为"阴性"、"anti-HBc(+)anti-HBs(+)"和"anti-HBe (+)anti-HBc(+)anti-HBs(+)",依次占18.231%(4 538/24 892)、9.911%(2 467/24 892)和4.395%(1 094/24 892);其他13种低频模式检出率为0.004%～0.992%."单anti-HBc(+)"和"anti-HBe(+)、anti-HBs(+)"两种模式在男女间的检出率差异有统计学意义(χ2值分别为7.143、8.123,P均＜0.01＝,其余15种模式性别间差异无统计学意义(P均＞0.05).共检出HBsAg阳性者94名,占0.38%.总anti-HBs阳性儿童19 899名,占79.94%,其中"单anti-HBs(+)"16 178名,占总anti-HBs阳性者的81.30%.单anti-HBs阳性率最高为＞1～2岁组(87.27%),最低为2 d～2周组(13.13%);16 178名儿童在3种抗体浓度组(10～100、＞100～1 000和＞1 000 mIU/ml)的比率依次为23.64%、27.66%和13.70%.总的anti-HBc(+)儿童有3 904名,占15.68%,阳性率最高为2～4周组(57.63%),最低为3～4岁组(3.46%).1 565名"阴性"模式儿童中,阴性率最低为9～12个月组(3.25%),最高为6～7岁组(36.21%).结论 本地区学龄前儿童中"单anti-HBs(+)"检出率最高,其抗体浓度多位于100～1 000 mIU/ml间;其次为"阴性"模式.HBsAg的检出率很低.乙肝模式检出率在本地区儿童男女间无差异,而与年龄相关.分析1岁内(主要为3个月内)儿童乙肝血清学模式应考虑母亲血清乙肝抗原抗体的影响.
目的 調查和分析浙江地區嬰幼兒和學齡前兒童乙型肝炎(簡稱乙肝)病毒血清學模式及其與年齡、性彆相關的分佈特徵,為噹地兒童乙肝的預防與控製提供依據.方法 採用電化學髮光技術在Roche e601免疫分析儀上檢測乙肝病毒血清學標誌物.應用實驗室信息管理繫統(LIS)將2006年7月至2010年3月期間所有乙肝病毒血清學檢測結果導齣為EXCEL數據文件,通過排除重複檢測者、年齡＞7週歲和住阯非浙江地區者,最終篩選齣24 892名研究對象,男15 810名,女9082名.採用SPSS 13.0和EXCEL 2003對乙肝病毒血清學檢測結果進行統計分析.結果 乙肝疫苗平均覆蓋率在浙江地區15 413名男孩和8 840名女孩中分彆為99.16%(15 413/15 543)和99.17%(8 840/8 914).所有24 892名7歲內兒童中檢齣乙肝病毒血清學模式17種,最主要模式為"單antiHBs(+)",佔65.997%(16 179/24 892);其次為"陰性"、"anti-HBc(+)anti-HBs(+)"和"anti-HBe (+)anti-HBc(+)anti-HBs(+)",依次佔18.231%(4 538/24 892)、9.911%(2 467/24 892)和4.395%(1 094/24 892);其他13種低頻模式檢齣率為0.004%～0.992%."單anti-HBc(+)"和"anti-HBe(+)、anti-HBs(+)"兩種模式在男女間的檢齣率差異有統計學意義(χ2值分彆為7.143、8.123,P均＜0.01＝,其餘15種模式性彆間差異無統計學意義(P均＞0.05).共檢齣HBsAg暘性者94名,佔0.38%.總anti-HBs暘性兒童19 899名,佔79.94%,其中"單anti-HBs(+)"16 178名,佔總anti-HBs暘性者的81.30%.單anti-HBs暘性率最高為＞1～2歲組(87.27%),最低為2 d～2週組(13.13%);16 178名兒童在3種抗體濃度組(10～100、＞100～1 000和＞1 000 mIU/ml)的比率依次為23.64%、27.66%和13.70%.總的anti-HBc(+)兒童有3 904名,佔15.68%,暘性率最高為2～4週組(57.63%),最低為3～4歲組(3.46%).1 565名"陰性"模式兒童中,陰性率最低為9～12箇月組(3.25%),最高為6～7歲組(36.21%).結論 本地區學齡前兒童中"單anti-HBs(+)"檢齣率最高,其抗體濃度多位于100～1 000 mIU/ml間;其次為"陰性"模式.HBsAg的檢齣率很低.乙肝模式檢齣率在本地區兒童男女間無差異,而與年齡相關.分析1歲內(主要為3箇月內)兒童乙肝血清學模式應攷慮母親血清乙肝抗原抗體的影響.
목적 조사화분석절강지구영유인화학령전인동을형간염(간칭을간)병독혈청학모식급기여년령、성별상관적분포특정,위당지인동을간적예방여공제제공의거.방법 채용전화학발광기술재Roche e601면역분석의상검측을간병독혈청학표지물.응용실험실신식관리계통(LIS)장2006년7월지2010년3월기간소유을간병독혈청학검측결과도출위EXCEL수거문건,통과배제중복검측자、년령＞7주세화주지비절강지구자,최종사선출24 892명연구대상,남15 810명,녀9082명.채용SPSS 13.0화EXCEL 2003대을간병독혈청학검측결과진행통계분석.결과 을간역묘평균복개솔재절강지구15 413명남해화8 840명녀해중분별위99.16%(15 413/15 543)화99.17%(8 840/8 914).소유24 892명7세내인동중검출을간병독혈청학모식17충,최주요모식위"단antiHBs(+)",점65.997%(16 179/24 892);기차위"음성"、"anti-HBc(+)anti-HBs(+)"화"anti-HBe (+)anti-HBc(+)anti-HBs(+)",의차점18.231%(4 538/24 892)、9.911%(2 467/24 892)화4.395%(1 094/24 892);기타13충저빈모식검출솔위0.004%～0.992%."단anti-HBc(+)"화"anti-HBe(+)、anti-HBs(+)"량충모식재남녀간적검출솔차이유통계학의의(χ2치분별위7.143、8.123,P균＜0.01＝,기여15충모식성별간차이무통계학의의(P균＞0.05).공검출HBsAg양성자94명,점0.38%.총anti-HBs양성인동19 899명,점79.94%,기중"단anti-HBs(+)"16 178명,점총anti-HBs양성자적81.30%.단anti-HBs양성솔최고위＞1～2세조(87.27%),최저위2 d～2주조(13.13%);16 178명인동재3충항체농도조(10～100、＞100～1 000화＞1 000 mIU/ml)적비솔의차위23.64%、27.66%화13.70%.총적anti-HBc(+)인동유3 904명,점15.68%,양성솔최고위2～4주조(57.63%),최저위3～4세조(3.46%).1 565명"음성"모식인동중,음성솔최저위9～12개월조(3.25%),최고위6～7세조(36.21%).결론 본지구학령전인동중"단anti-HBs(+)"검출솔최고,기항체농도다위우100～1 000 mIU/ml간;기차위"음성"모식.HBsAg적검출솔흔저.을간모식검출솔재본지구인동남녀간무차이,이여년령상관.분석1세내(주요위3개월내)인동을간혈청학모식응고필모친혈청을간항원항체적영향.
Objective To investigate the serological patterns of hepatitis B, understand the prevalence of hepatitis B infection in neonates, infants and preschool children based on hospital data in Zhejiang province and provide a basis for prevention and control of local pediatric hepatitis B infection. Methods Five serological markers were detected on electrochemiluminescence analyzer Roche E601. The results of all serological markers in children with hepatitis B from 2006 to 2010 were exported from laboratory information system and 24 892 (15 810 boys and 9 082 girls) cases were selected for further analysis according to the exclusion criterion including duplicate analysis, children older than 7 years and children living out of Zhejiang province. SPSS13.0 software and EXCEL 2003 were employed for statistical analysis in this study. Results Average HBV vaccination coverage rates among 15 413 boys and 8 840 girls were 99. 16%(98. 87% -99. 45%) and 99. 17% (98. 29% -99. 53%), respectively. Seventeen serological patterns of hepatitis B were observed, and "anti-HBs(+)alone"(64. 997%) was the dominant patterns followed by "negative" (18. 231%), "anti-HBc(+)anti-HBs(+)"(9.911%) and "anti-HBe(+)antiHBc(+) anti-HBs(+)"(4.395%), and the positive rates for other patters were 0. 004% -0. 992%. There were significant difference of detection rates for "anti-HBc(+)alone" and "anti-HBe(+)anti-HBs(+)"patterns between male and female (χ2=7.143,8.123, all P＜0.01), and there were no significant difference of detection rates for the other 15 patterns between male and female (all P ＞0.05). Ninety-four HBsAg positive cases were detected, accounting for 0.38%. Totally 19 899 anti-HBs positive cases were detected, accounting for 79.94%. Totally 16 187 cases were "anti-HBs(+) alone", accounting for 81.3%of the total anti-HBs(+) cases. The group of 1-2 years and 2 day-2 week had the highest and lowest rate for anti-HBs(+)alone respectively. The ratios in three groups with different anti-HBs levels (10-100 mIU/ml,100-1000 mIU/ml and ＞ 1000 mIU/ml) in 16 178 anti-HBs(+) children were 23. 64%, 27.66% and 13. 70%, respectively. Totally 3 904 cases with anti-HBc(+) were detected, accounting for 15.68%. The highest positivity rate and lowest positivity rate were in the group of 2-4 week and 3-4 week respectively. In total 1 565 cases with negative results, the lowest negativity rate and highest negativity rate were in the group of 9-12 month (3. 25%) and 6-7 age (36. 21%) respectively. Conclusions The dominant serological patterns of hepatitis B in local young children is "anti-HBs (+) alone" and most of the anti-HBs concentrations ranged from 100 to 1000 mIU/ml, followed by "negative". The total positive rate of HBsAg is mild. The rate of hepatitis B serological patterns in children is associated with ages but independent of gender. For interpretation of serological patterns of HBV in children below 12 months (especially in 3 months) the status of their mothers' HBV infection should also be taken into consideration.
