CAJ | 학술논문

以4种苹果砧木幼苗为试材,利用高效液相色谱法(HPLC)测定根系分泌酚类物质的含量,并比较不同砧木品种间根系分泌物成分的差异.结果表明:采用C18分离柱,波长280 nm,流动相为乙腈和水进行梯度洗脱,流速1 mL/min,分离效果良好.在一定浓度范围内,10种酚类物质的峰面积与浓度间存在显著的线性关系.不同苹果砧木根系分泌物种类和数量不同,主要成分为间苯三酚、根皮素、根皮苷和苯甲酸,分泌物含量从高到低顺序依次为'怀来海棠'、'新疆海棠'、'莱芜难咽'、'平邑甜茶'.
이4충평과침목유묘위시재,이용고효액상색보법(HPLC)측정근계분비분류물질적함량,병비교불동침목품충간근계분비물성분적차이.결과표명:채용C18분리주,파장280 nm,류동상위을정화수진행제도세탈,류속1 mL/min,분리효과량호.재일정농도범위내,10충분류물질적봉면적여농도간존재현저적선성관계.불동평과침목근계분비물충류화수량불동,주요성분위간분삼분、근피소、근피감화분갑산,분비물함량종고도저순서의차위'부래해당'、'신강해당'、'래무난인'、'평읍첨다'.
The main phenolic compounds in root exudates of apple rootstock seedlings were identified and qualified using high-performance liquid chromatography (HPLC). The results showed that the optimal chromatographic separation was achieved on a reverse phase C18 column with acetonitrile-water as the mobile phase using gradient elution, at 280 nm, with the flow rate of 1 mL/min. The peak area was linear with the phenolic concentration in the range of 0.01-1.9 μg/g. The main phenolic compounds were phloroglucinol, phloretin, phloridzin, and benzoic acid. The contents of detected phenolic compounds, in a descending order, in root exudates of apple rootstocks was as follows: huailaihaitang (Malus micromalus Makino.)> xinjianghaitang (Malus sieversii Roem)>laiwunanyan (Malus micromalus M) > pingyitiancha (Malus hepenhensis Rehd.).