CAJ | 학술논문

以二氢槲皮素的得率为指标,采用超声-微波交替法从落叶松中提取二氢槲皮素.以体积分数60%乙醇作为提取溶剂,料液比1:12,浸润3 h,强档功率提取1次,微波提取10 min,超声提取40 min,超声-微波交替提取的条件为超声提取40 min后再微波提取10 min.二氢槲皮素单独超声和微波提取时得率分别为0.034%和0.074%,超声-微波交替提取时得率可达0.12%,明显优于单独超声或微波提取.通过扫描电子显微镜观察超声提取、微波提取和超声-微波交替提取所得的物料,超声-微波交替提取的抽提效果最为明显.
이이경곡피소적득솔위지표,채용초성-미파교체법종락협송중제취이경곡피소.이체적분수60%을순작위제취용제,료액비1:12,침윤3 h,강당공솔제취1차,미파제취10 min,초성제취40 min,초성-미파교체제취적조건위초성제취40 min후재미파제취10 min.이경곡피소단독초성화미파제취시득솔분별위0.034%화0.074%,초성-미파교체제취시득솔가체0.12%,명현우우단독초성혹미파제취.통과소묘전자현미경관찰초성제취、미파제취화초성-미파교체제취소득적물료,초성-미파교체제취적추제효과최위명현.
An ultrasonic-microwave altemant method for extracting dihydroquercetin from larch wood was applied by using dihydroquercetin yield as an index. The extraction was conducted using 60% ethanol as solvent, the solid to liquid ratio of 1 : 12, and soakage time of 3 hours. The microwave treatment time was 10 min, ultrasonic treatment time was 40 min respectively and the extraction was performed one time with the strongest power. The ultrasonic-microwave alternant extraction was performed by ultrasonic extraction for 40 min and then microwave extraction for 10min. The yield of dihydroquercetin with ultrasonic and microwave extraction were 0.034% and 0.074% respectively, while that of ultrasonic-microwave alternant extraction was 0.12% which is much higher than ultrasonic and microwave extraction alone. By scanning electron microscopy (SEM) observation, the ultrasonic-microwave altemant effect of substance dissolution from plant cell was the most obvious among the above-mentioned three methods.