CAJ | 학술논문

目的:探讨环氧合酶-2(cyclooxygenase-2,COX-2)选择性抑制剂NS-398对人肝癌BEL-7402细胞凋亡及凋亡抑制蛋白survivin、XIAP和c-IAP1表达的调节作用.方法:用不同浓度的NS-398作用BEL-7402细胞后,MTT法测定细胞增殖抑制情况,FCM法和TUNEL法检测细胞凋亡情况,免疫细胞化学法检测COX-2、survivin、XIAP和c-IAP1蛋白的表达情况.结果:NS-398 可以显著抑制BEL-7402细胞的增殖,诱导其凋亡.免疫细胞化学法检测结果显示,与未处理组相比,NS-398作用可使BEL-7402细胞中COX-2、survivin、XIAP和c-IAP1蛋白的表达明显下调(P<0.01).结论:NS-398对人肝癌细胞株BEL-7402有抑制细胞增殖和诱导细胞凋亡的作用,其机制可能与通过下调survivin、XIAP和c-IAP1的表达有关.
목적:탐토배양합매-2(cyclooxygenase-2,COX-2)선택성억제제NS-398대인간암BEL-7402세포조망급조망억제단백survivin、XIAP화c-IAP1표체적조절작용.방법:용불동농도적NS-398작용BEL-7402세포후,MTT법측정세포증식억제정황,FCM법화TUNEL법검측세포조망정황,면역세포화학법검측COX-2、survivin、XIAP화c-IAP1단백적표체정황.결과:NS-398 가이현저억제BEL-7402세포적증식,유도기조망.면역세포화학법검측결과현시,여미처리조상비,NS-398작용가사BEL-7402세포중COX-2、survivin、XIAP화c-IAP1단백적표체명현하조(P<0.01).결론:NS-398대인간암세포주BEL-7402유억제세포증식화유도세포조망적작용,기궤제가능여통과하조survivin、XIAP화c-IAP1적표체유관.
Objective:To investigate the effect and elucidate the mechanism of the selective cyclooxygenase-2(COX-2)inhibitor NS-398 on apoptosis and survivin, XIAP and c-IAP1 expressions of hepatocarcinoma cell lines. Methods:The proliferation of hepatocarcinoma BEL-7402 cells treated with NS-298 at different concentrations were evaluated by MTT assay. The apoptosis was detected by flow cytometry (FCM) and TUNEL assay. Expressions of COX-2, survivin, XIAP and c-IAP1 were analyzed by immunocytochemical staining. Results: NS-398 significantly inhibited cell proliferation of BEL-7402 cells and induced apoptosis. Immunocytochemisty indicated that the expressions of COX-2, survivin, XIAP and c-IAP1 were significantly down-regulated in BEL-7402 cells by NS-398 treatment compared with untreatment group (P<0.01). Conclusion:NS-398 inhibits the proliferation and induced apoptosis of BEL-7402 cells. The mechanism may be related with down-regulation of the survivin, XIAP and c-IAP1 expression.