CAJ | 학술논문

目的研究神经生长因子在小鼠不同周龄睾丸组织中的定量和定位表达.方法分别剖取不同周龄雄性小鼠的睾丸组织,部分提取总RNA,real-time PCR相对定量分析神经生长因子mRNA的表达量;另外部分组织固定、包埋,进行SABC法免疫组化分析,以观察神经生长因子蛋白在各周睾丸组织中的定位.结果 Real-timePCR定量分析表明:小鼠生后1周龄睾丸组织有神经生长因子mRNA的表达,生后3周龄表达量达峰值,5周之后随鼠龄的增加呈下降趋势,成年小鼠睾丸组织的神经生长因子mRNA表达维持在一定水平.免疫组化定位分析显示:睾丸组织的神经生长因子蛋白表达于小鼠出生后的各个时期内,1周龄睾丸组织免疫阳性反应主要位于支持细胞,精原细胞也有着色;3周龄睾丸组织的间质细胞、各级生精细胞、支持细胞、管周肌样细胞表达均呈现阳性;5周后的睾丸组织内神经生长因子呈低水平表达,主要表达于间质细胞和生精细胞内.结论神经生长因子mRNA的表达量随着小鼠睾丸的生长发育期存在着一定的规律性变化;神经生长因子蛋白的表达在小鼠睾丸生长发育的不同时期其主要表达部位不同.
목적 연구신경생장인자재소서불동주령고환조직중적정량화정위표체.방법 분별부취불동주령웅성소서적고환조직,부분제취총RNA,real-time PCR상대정량분석신경생장인자mRNA적표체량;령외부분조직고정、포매,진행SABC법면역조화분석,이관찰신경생장인자단백재각주고환조직중적정위.결과 Real-timePCR정량분석표명:소서생후1주령고환조직유신경생장인자mRNA적표체,생후3주령표체량체봉치,5주지후수서령적증가정하강추세,성년소서고환조직적신경생장인자mRNA표체유지재일정수평.면역조화정위분석현시:고환조직적신경생장인자단백표체우소서출생후적각개시기내,1주령고환조직면역양성반응주요위우지지세포,정원세포야유착색;3주령고환조직적간질세포、각급생정세포、지지세포、관주기양세포표체균정현양성;5주후적고환조직내신경생장인자정저수평표체,주요표체우간질세포화생정세포내.결론 신경생장인자mRNA적표체량수착소서고환적생장발육기존재착일정적규률성변화;신경생장인자단백적표체재소서고환생장발육적불동시기기주요표체부위불동.
Objective To study the location and the expression of nerve growth factor(NGF)in mouse testes at different developmental stages.Methods The testes were taken out from mice at different developmental stages,respectively.Parts of the testis tissue were used to extract total RNA and real-time PCR was used to detect the expression of NGF.The rest tissues were fixed and embedded in paraffin.SABC immunohistochemical staining was used to observe the location of NGF in the testes. Results Reat-time PCR results showed that NGF mRNA could be detected in the testes of mouse at postnatal 1 st week and its expression level increased with the development,and reached the highest point at 3rd week.After 5 weeks it lowered down to a stable level.Immunohistochemistry showed that NGF expressed in the mouse testes at all developmental stages.At postnatal 1 st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining.NGF positive staining in the testes was observed in interstitial cells,spermatogenetic cells,sustentacular cells and Leydig cells at 3rd week.After the postnatal 5th week,NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells,but the intensity of reaction was weaker than that at 1 st and 3rd weeks.Conclusion The expression levels of NGF in mouse testes show regular changes and the expression profile and location of NGF in the testes are different at different developmental stages.