CAJ | 학술논문

目的:研究贲门腺癌(gastric cardia adenocarcinoma,GCA)中分泌型卷曲相关蛋白4(secreted frizzled related protein 4,SFRP4)及SFRP5基因启动子区甲基化状态与贲门腺癌发生发展的关系.方法:采用甲基化特异性PCR(methylation specific PCR,MSP)方法检测94例GCA组织及47例相应癌旁正常组织中SFRP4和SFRP5基因的甲基化情况,并分析其与临床病理特征之间的关系.结果:94例GCA组织中SFRP4基因甲基化率为68.1%(64/94),显著高于癌旁正常组织中的甲基化发生率8.5%(4/47)(P<0.01);SFRP5基因在贲门腺癌组织中的甲基化发生率为79.8%(75/94),显著高于癌旁正常组织中的甲基化发生率12.8%(16/47)(P<0.01).SFRP4基因在高、中分化腺癌组甲基化发生率为35%(14/40),显著低于低分化腺癌组的发生率92.6%(50/54)(P<0.01);SFRP4基因在无淋巴结转移组的甲基化发生率为60.5%(23 38),低于有淋巴结转移组73.2%(41 56).但其差异无统计学意义(P>0.05).SFRP5基因在低分化腺癌组甲基化发生率为85.1%(46/54),高于高、中分化腺癌组甲基化发生率72.5%(29/40),但其差异无统计学意义(P>0.05);SFRP5基因甲基化发生率在无淋巴结转移组为65.8%(25/38),显著低于有淋巴结转移组89.3%(50/56)(P<0.05).57例贲门腺癌组织中SFRP4和SFRP5基因同时发生甲基化者,高、中分化18例,低分化39例,两者间的差异具有统计学意义(P<0.05).结论:SFRP4和SFRP5基因可能参与了贲门腺癌的发生,SFRP4,SFRP5基因的高甲基化状态与贲门腺癌的恶性行为有关.
목적:연구분문선암(gastric cardia adenocarcinoma,GCA)중분비형권곡상관단백4(secreted frizzled related protein 4,SFRP4)급SFRP5기인계동자구갑기화상태여분문선암발생발전적관계.방법:채용갑기화특이성PCR(methylation specific PCR,MSP)방법검측94례GCA조직급47례상응암방정상조직중SFRP4화SFRP5기인적갑기화정황,병분석기여림상병리특정지간적관계.결과:94례GCA조직중SFRP4기인갑기화솔위68.1%(64/94),현저고우암방정상조직중적갑기화발생솔8.5%(4/47)(P<0.01);SFRP5기인재분문선암조직중적갑기화발생솔위79.8%(75/94),현저고우암방정상조직중적갑기화발생솔12.8%(16/47)(P<0.01).SFRP4기인재고、중분화선암조갑기화발생솔위35%(14/40),현저저우저분화선암조적발생솔92.6%(50/54)(P<0.01);SFRP4기인재무림파결전이조적갑기화발생솔위60.5%(23 38),저우유림파결전이조73.2%(41 56).단기차이무통계학의의(P>0.05).SFRP5기인재저분화선암조갑기화발생솔위85.1%(46/54),고우고、중분화선암조갑기화발생솔72.5%(29/40),단기차이무통계학의의(P>0.05);SFRP5기인갑기화발생솔재무림파결전이조위65.8%(25/38),현저저우유림파결전이조89.3%(50/56)(P<0.05).57례분문선암조직중SFRP4화SFRP5기인동시발생갑기화자,고、중분화18례,저분화39례,량자간적차이구유통계학의의(P<0.05).결론:SFRP4화SFRP5기인가능삼여료분문선암적발생,SFRP4,SFRP5기인적고갑기화상태여분문선암적악성행위유관.
OBJECTIVE: We investigated the promoter methylation of secreted frizzled-related protein 4(SFRP4) and SFRP5 gene in gastric eardia adenoeareinoma (GCA). METHODS: Methylation specific PCR (MSP) method was used to examine the methylation status of the 5' CpG island of SFRP4 and SFRP5 genes in 94 tumors and 47 corresponding normal tissues. RESULTS: Methylation frequencies of SFRP4 and SFRP5 genes in tumors were 68.1% (64/ 94) and 79.8% (75/94), respectively, significantly higher than that in corresponding normal tissues (8.5% and 12.8%, respectively) (P < 0.01). Methylation frequencies of SFRP4 in poor differentiation group (92.6% ,50/54)was significantly higher than that in moderate and poor-moderate differentiation groups (35%, 14/40). Methylation frequencies of SFRP5 in lymph node metastasis group (89.3%, 50/56) was significantly higher than that in no lymph node metastasis group (65.8%,25/38) . Methylation frequencies of SFRP5 in poor differentiation group was higher than that in moderate and poor-moderate differentiation groups, and SFRP4 in lymph node metastasis group was higher than that in no lymph node metastasis group but without significant difference(P> 0.05). 57 cases of GCA showed simultaneous methylation of SFRP4 and SFRP5 genes, including 18 moderate and poor-moderate differentiation tumors, and 39 moderate differentiation tumors. Simultaneous methylation frequencies of SFRP4 and SFRP5 genes in moderate and poor-moderate differentiation groups was significantly lower than that in moderate group. CONCLUSION: SFRP4 and SFRP5 genes might be associated with oncogenanesis of GCA and hypermethylation of these genes might be related to the malignant behavior of GCA.