时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
4期
993-995
,共3页
金钗石斛%组培苗%直接扩增长度多态性%遗传稳定性%指纹图谱
金釵石斛%組培苗%直接擴增長度多態性%遺傳穩定性%指紋圖譜
금차석곡%조배묘%직접확증장도다태성%유전은정성%지문도보
Dendrobium nobile%Tissue culture seedling%DALP%Genetic stability%Fingerprinting
目的 研究珍稀药用金钗石斛DNA指纹图谱,初步探讨直接扩增长度多态性(DALP)分子标记技术在石斛组织培养过程中遗传稳定性检测上的应用.方法 采用直接扩增长度多态性(DALP)技术对金钗石斛野生移栽苗和组培苗进行基因组DNA多态性分析.结果 从12对引物组合中筛选出6对能获清晰条带的引物组合,分别利用琼脂糖和聚丙烯酰胺对DALP-PCR产物进行凝胶电泳,发现两组苗扩增的DNA带型基本一致,未发现明显变异,说明金钗石斛茎尖离体继代培养具有较高的遗传稳定性.结论 DALP分子标记技术可用于石斛遗传稳定性和遗传多样性研究.
目的 研究珍稀藥用金釵石斛DNA指紋圖譜,初步探討直接擴增長度多態性(DALP)分子標記技術在石斛組織培養過程中遺傳穩定性檢測上的應用.方法 採用直接擴增長度多態性(DALP)技術對金釵石斛野生移栽苗和組培苗進行基因組DNA多態性分析.結果 從12對引物組閤中篩選齣6對能穫清晰條帶的引物組閤,分彆利用瓊脂糖和聚丙烯酰胺對DALP-PCR產物進行凝膠電泳,髮現兩組苗擴增的DNA帶型基本一緻,未髮現明顯變異,說明金釵石斛莖尖離體繼代培養具有較高的遺傳穩定性.結論 DALP分子標記技術可用于石斛遺傳穩定性和遺傳多樣性研究.
목적 연구진희약용금차석곡DNA지문도보,초보탐토직접확증장도다태성(DALP)분자표기기술재석곡조직배양과정중유전은정성검측상적응용.방법 채용직접확증장도다태성(DALP)기술대금차석곡야생이재묘화조배묘진행기인조DNA다태성분석.결과 종12대인물조합중사선출6대능획청석조대적인물조합,분별이용경지당화취병희선알대DALP-PCR산물진행응효전영,발현량조묘확증적DNA대형기본일치,미발현명현변이,설명금차석곡경첨리체계대배양구유교고적유전은정성.결론 DALP분자표기기술가용우석곡유전은정성화유전다양성연구.
Objective To study DNA fingerprint of Dendrobium nobile and application of direct amplification of length polymorphism (DALP) to the identification of genetic stability on the course of tissue culture.Methods The polymorphism of transplanting wild seedlings and tissue culture seedlings from D.nobile were detected by DALP technique.Results Six pairs of primer combinations screened from 12 pairs of primer combinations might amplify clear bands.By agarose and polyacrylamide gel electrophoresising,respectively,the DNA bandtypes of DALP-PCR products were basic consistent and the obvious variation were not shown.It indicated that the genetic stability was high on the in vitro shoot tip subculture of D.nobile.Conclusion DALP molecular marker technology can be used for the research of the genetic stability and genetic diversity on Dendrobium.
