CAJ | 학술논문

以秦皇岛海域的缢蛏为原料,通过不同提取方法的比较,确定超声辅助酶解提取法为较优的多糖提取方法,并通过响应面法对缢蛏多糖提取工艺进行优化.试验结果表明,原料超声预处理30 min后再进行酶解提取,在添加3.99%的木瓜蛋白酶、50.9 ℃、酶提8.94 h条件下缢蛏多糖提取量达9.41 mg/g(鲜).该方法对研究海水贝类多糖功能性产品原料的选取及产品的开发与应用有重要意义.
이진황도해역적액정위원료,통과불동제취방법적비교,학정초성보조매해제취법위교우적다당제취방법,병통과향응면법대액정다당제취공예진행우화.시험결과표명,원료초성예처리30 min후재진행매해제취,재첨가3.99%적목과단백매、50.9 ℃、매제8.94 h조건하액정다당제취량체9.41 mg/g(선).해방법대연구해수패류다당공능성산품원료적선취급산품적개발여응용유중요의의.
Razor clam polysaccharide with various biological activity was rich in polysaccharide functional product. Establishment of more economical and reasonable experimental design through maths model,it was very important signification for exploitation and application of razor clam polysaccharide. The research carried out in the Qinhangdao sea showed that the supersonic assistance enzymolysis extraction was found to be the preferable polysaccharide extraction method for the razor clam polysaccharide extraction by the response surface method. The results indicated that the feasible extraction condition was as the following: 30 min of supersonic assistance extract time was,amount of papain of 3.99%,extract Temperature of 50.9 ℃,extract time of 8.94 h. Under this condition the razor clam polysaccharide extraction content was achieved at a rate of the 9.41 mg/g fresh weight. This research has the vital significance regarding the marine shellfish polysaccharide function product raw material selection and the product development and the application.