CAJ | 학술논문

背景:以往研究显示,酸性成纤维细胞因子(acidic fibroblast growth factor,aFGF)复合部分脱蛋白骨(partially deproteinised bone,PDPB)对实验动物早期股骨头缺血坏死血管再生具有良好的促进作用,但其组织学变化尚不明确.目的:从组织学变化角度观察aFGF复合PDPB修复兔早期股骨头缺血坏死的效果.方法:用新西兰大白兔制备早期股骨头缺血坏死动物模型,建模后随机分为空白组、PDPB组和aFGF/PDPB组.PDPB组植入PDPB,aFGF/PDPB组植入aFGF/PDPB.所有动物分别于第2,4,8周取材进行苏木精伊红染色观察成骨情况.结果与结论:空白组第8周缺损区被纤维结缔组织填充,在交界处有少量骨样组织形成.PDPB组第4周有少量新骨生成,髓腔形成,第8周较多植入物吸收,髓腔形成,有很多成骨细胞及髓细胞分布其中.aFGF/PDPB组各时间点成骨都普遍优于PDPB组,4周组移植物腔填充以骨样组织,可见较多的成骨前体细胞和骨母细胞,可见较多的微血管,骨样组织开始重建.第8周植入物已被新骨取代,髓腔已形成具有很多骨髓细胞分布其中,骨小梁交界处有大量成骨细胞,同时有少量破骨细胞存在可能参与骨塑形,骨陷窝可见成熟骨细胞.组织学检测结果提示,在修复股骨头缺血坏死的效果上,aFGF复合PDPB优于单独应用PDPB.
배경:이왕연구현시,산성성섬유세포인자(acidic fibroblast growth factor,aFGF)복합부분탈단백골(partially deproteinised bone,PDPB)대실험동물조기고골두결혈배사혈관재생구유량호적촉진작용,단기조직학변화상불명학.목적:종조직학변화각도관찰aFGF복합PDPB수복토조기고골두결혈배사적효과.방법:용신서란대백토제비조기고골두결혈배사동물모형,건모후수궤분위공백조、PDPB조화aFGF/PDPB조.PDPB조식입PDPB,aFGF/PDPB조식입aFGF/PDPB.소유동물분별우제2,4,8주취재진행소목정이홍염색관찰성골정황.결과여결론:공백조제8주결손구피섬유결체조직전충,재교계처유소량골양조직형성.PDPB조제4주유소량신골생성,수강형성,제8주교다식입물흡수,수강형성,유흔다성골세포급수세포분포기중.aFGF/PDPB조각시간점성골도보편우우PDPB조,4주조이식물강전충이골양조직,가견교다적성골전체세포화골모세포,가견교다적미혈관,골양조직개시중건.제8주식입물이피신골취대,수강이형성구유흔다골수세포분포기중,골소량교계처유대량성골세포,동시유소량파골세포존재가능삼여골소형,골함와가견성숙골세포.조직학검측결과제시,재수복고골두결혈배사적효과상,aFGF복합PDPB우우단독응용PDPB.
BACKGROUND: Previous studies have demonstrated that acidic fibroblast growth factor (aFGF) combined with partially deproteinized bone (PDPB) (aFGF/PDPB) well promotes avascularization in animals with early-stage avascular necrosis of the femoral head (ANFH), but the histological results remain unknown.OBJECTIVE: To observe the histological repairing effects of aFGF/PDPB on early-stage ANFH in rabbits. METHODS: New Zealand rabbits were established models of bilateral ANFH and were randomly divided into a blank group, a simple PDPB group, and an aFGF/PDPB group. PDPB and aFGF/PDPB bone were implanted into the PDPB and aFGF/PDPB group accordingly. The blank group did not receive any implantation. At 2, 4, and 8 weeks after surgery, all animals were sacrificed for histological examination to observe the osteogenesis by hematoxylin-eosin staining.RESULTS AND CONCLUSION: Defects were filled with granulation tissues and fibrous connective tissues, only a little osteoid tissue formed at the borderline in the blank group at the end of the 8th week. In the PDPB group, a little new bone and cavitas medullaris formed. At 8 weeks, lots of graft was absorbed and cavitas medullaris formed with more osteoplasts and myeloid cells in it. The osteogenesis in the aFGF/PDPB group was better than that of PDPB group in each time point. At 4 weeks, the transplanted cavity was filled with osteoid tissues, a lot of osteogenic precursor cells and osteoblasts could be seen. Plenty of micrangium was observed, and osteoid tissues began to rebuild. At 8 weeks, the graft was replaced by bone tissues, and cavitas medullaris were formed with lots of bone marrow cells in it. At the borderline of the bone trabecula, there were lots of osteoplast and little osteoclasts, which may play a role in bone remodeling. There were mature bone cells in bone lacuna. Results indicate that aFGF/PDPB has better repair effect on rabbit model of ANFH than that of simple PDPB.