CAJ | 학술논문

"肿瘤干细胞"学说的提出为肿瘤包括乳腺肿瘤在内的治疗提供了新的思路.乳腺肿瘤干细胞正确的分选纯化是研究的关键,其分选主要历经侧群细胞分选、无血清悬浮培养、细胞表面分子标记分选、ALDEFLUOR试剂盒检测乙醛脱氢酶1高活性分选法4个阶段;由于化疗会富集肿瘤干细胞,部分学者把化疗耐药也作为一种分选的方法.目前有关乳腺肿瘤干细胞分选结果的争论主要集中在标志物CD44+ CD24-、ALDH1+ 之间.哪种分选方法或标志物的选择更适宜分选乳腺肿瘤干细胞成为亟待解决的问题.
"종류간세포"학설적제출위종류포괄유선종류재내적치료제공료신적사로.유선종류간세포정학적분선순화시연구적관건,기분선주요력경측군세포분선、무혈청현부배양、세포표면분자표기분선、ALDEFLUOR시제합검측을철탈경매1고활성분선법4개계단;유우화료회부집종류간세포,부분학자파화료내약야작위일충분선적방법.목전유관유선종류간세포분선결과적쟁론주요집중재표지물CD44+ CD24-、ALDH1+ 지간.나충분선방법혹표지물적선택경괄의분선유선종류간세포성위극대해결적문제.
In recent years, the theory of cancer stem cells has provided a new perspective on the treatment of cancers including breast cancer. The accurate sorting of breast cancer stem cells is critical. The sorting procedure is consist of 4 steps: isolation of side population, serum-free suspension culture,determination of specific cell surface markers and of the activity of aldehyde dehydrogenase 1 ( ALDH1 ) enzymatic through the ALDEFLUOR assay. Some studies choose drug resistance as an additional method for sorting cancer stem cells because of the enrichment of cancer stem cells after chemotherapy . The controversy about the sorting outcome of breast cancer stem cell mainly focus on molecular markers like CD44+ CD24- and ALDH1+ . The problem needed to be settled is to identify which sorting method and markers are appropriate.