CAJ | 학술논문

目的研究3种目前国内常用的器官保存液对供肝细胞凋亡的影响.方法分别用UW液、HG-A液和WMO-1号液灌洗并保存大鼠肝脏,于保存后0、12、24 h用原位末端标记法检测供肝细胞凋亡情况,并将保存24 h的肝脏行大鼠原位全肝移植,观察受者的3 d存活率.结果 3种保存液保存的肝脏均在保存12 h时出现细胞凋亡,HC-A液组与WMO-1号液组的凋亡指数(AI)高于UW液组(P＜0.001),而HC-A液组与WMO-1号液组比较,差异不显著(P＞0.05);UW液组仅在保存24 h并行肝移植后AI才明显上升,其大鼠肝移植后3 d存活率为3/5,而HGA液组及WMO-1号液组的3 d存活率为0.结论引起移植肝细胞凋亡的关键因素是冷缺血后的有氧再灌注,这是移植物早期丧失功能的主要原因;细胞凋亡及器官保存液所造成的细胞损伤也是不可忽视的重要因素.
목적 연구3충목전국내상용적기관보존액대공간세포조망적영향.방법 분별용UW액、HG-A액화WMO-1호액관세병보존대서간장,우보존후0、12、24 h용원위말단표기법검측공간세포조망정황,병장보존24 h적간장행대서원위전간이식,관찰수자적3 d존활솔.결과 3충보존액보존적간장균재보존12 h시출현세포조망,HC-A액조여WMO-1호액조적조망지수(AI)고우UW액조(P＜0.001),이HC-A액조여WMO-1호액조비교,차이불현저(P＞0.05);UW액조부재보존24 h병행간이식후AI재명현상승,기대서간이식후3 d존활솔위3/5,이HGA액조급WMO-1호액조적3 d존활솔위0.결론 인기이식간세포조망적관건인소시랭결혈후적유양재관주,저시이식물조기상실공능적주요원인;세포조망급기관보존액소조성적세포손상야시불가홀시적중요인소.
Objective To study the effect of 3 different organ storage solutions commonly used on donor hepatocytic apoptosis.Methods The donor livers were separately flushed and preserved for 24 hwith UW,HC-A and WMO-1 solutions.The orthotopic liver transplantation in(OLT)rats were performed and the survival rate within 3-days were observed.The apoptosis of donor liver in different time points of 3 storage solutions was detected by in situ terminal labeling technique(TUNEL method).Results The apoptosis occurred 12 h after the liver preservation with 3 storage solutions.The apoptotic index(AI)in HC-A and WMO-1 groups WaS higher than Uw group(P＜0.001).In UW preserved group,the AI was increased rapidly following warm oxygenated reperfusion of liver graft preserved in UW solution for 24 h after transplantation.3-days survival rate of OLT rats in UW group was 60%.significantly higher than in HGA and WMO-1 groups(P＜0.01).Conclusions There might be some difference in the constitution between the UW solution and HC-A or WMO-1 solutions.The critical factor that caused the apoptosis of transplanted liver cell might be the oxygenated reperfusion following the cold ischemia,and that was the main reason for primary graft nonfunction following liver transplantation.But the injury to hepatocytes caused by apoptosis and difference of organ storage solutions was also one of the important factors that couldn't be neglected.