CAJ | 학술논문

目的探讨氢气饱和生理盐水对百草枯(PQ)中毒大鼠肺损伤及肺纤维化的保护作用.方法该实验在南昌大学第一附属医院动物实验室完成.48只SD大鼠随机分为对照组、染毒组和干预组,每组16只.染毒组及干预组给予50 mg/kgPQ一次性灌胃染毒,对照组给予等量蒸馏水灌胃.干预组大鼠在染毒1 h后开始予腹腔注射氧气饱和生理盐水5 mL/kg,2次/d,直至处死;染毒组及对照组大鼠按体质量给予等量生理盐水腹腔内注射.于染毒第3天及第21天分别测定各组大鼠动脉血氧分压(PaO2)、肺组织8-羟基脱氧鸟苷(8-OHDG)含量及转化生长因子β1(TGF-31)的表达.结果 (1)染毒后3 d,与对照组比较,染毒组及干预组PaO2均明显下降(P＜0.01);与染毒组比较,干预组PaO2有所改善(P＜0.05).21 d后,与对照组比较,染毒组PaO2仍明显减低(P＜0.01),干预组有一定程度下降(P＜0.05);与染毒组比较,干预组PaO2明显升高(P＜0.01).(2)染毒后3 d,与对照组比较,染毒组8-OHDG含量明显增加(P＜0.01),干预组亦有一定程度增加(P＜0.05);与染毒组比较,干预组8-OHDG含量有明显下降(P＜0.01).21 d后,各组间比较差异无统计学意义(P＞0.05).(3)染毒后3 d,与对照组比较,染毒组TGF-β1表达明显增加(P＜0.01),干预组有一定程度增加(P＜0.05);干预组较染毒组间比有一定下降(P＜0.05).21 d后,与对照组比较,染毒组TGF-β1表达明显增加(P＜0.01),干预组有一定增加(P＜0.05);与染毒组比较,干预组TGF-β1表达明显下降(P＜0.01).结论氢气饱和生理盐水能改善PQ大鼠氧化应激状态,减轻肺氧化损伤,降低肺纤维化程度.
목적 탐토경기포화생리염수대백초고(PQ)중독대서폐손상급폐섬유화적보호작용.방법 해실험재남창대학제일부속의원동물실험실완성.48지SD대서수궤분위대조조、염독조화간예조,매조16지.염독조급간예조급여50 mg/kgPQ일차성관위염독,대조조급여등량증류수관위.간예조대서재염독1 h후개시여복강주사양기포화생리염수5 mL/kg,2차/d,직지처사;염독조급대조조대서안체질량급여등량생리염수복강내주사.우염독제3천급제21천분별측정각조대서동맥혈양분압(PaO2)、폐조직8-간기탈양조감(8-OHDG)함량급전화생장인자β1(TGF-31)적표체.결과 (1)염독후3 d,여대조조비교,염독조급간예조PaO2균명현하강(P＜0.01);여염독조비교,간예조PaO2유소개선(P＜0.05).21 d후,여대조조비교,염독조PaO2잉명현감저(P＜0.01),간예조유일정정도하강(P＜0.05);여염독조비교,간예조PaO2명현승고(P＜0.01).(2)염독후3 d,여대조조비교,염독조8-OHDG함량명현증가(P＜0.01),간예조역유일정정도증가(P＜0.05);여염독조비교,간예조8-OHDG함량유명현하강(P＜0.01).21 d후,각조간비교차이무통계학의의(P＞0.05).(3)염독후3 d,여대조조비교,염독조TGF-β1표체명현증가(P＜0.01),간예조유일정정도증가(P＜0.05);간예조교염독조간비유일정하강(P＜0.05).21 d후,여대조조비교,염독조TGF-β1표체명현증가(P＜0.01),간예조유일정증가(P＜0.05);여염독조비교,간예조TGF-β1표체명현하강(P＜0.01).결론 경기포화생리염수능개선PQ대서양화응격상태,감경폐양화손상,강저폐섬유화정도.
Objective To investigate protective effects of the hydrogen saturated saline on acute lung injury and pulmonary fibrosis in rats with paraquat (PQ) poisoning. Method Forty-eight Sprague-Dawley rats were randomly divided into 3 groups, namely control group, PQ poisoning group and intervention group (n = 16 rats in each group) . Animals in PQ poisoning group and intervention group were fed with PQ in dosage of 50 mg / kg. Rats of control group were fed equivalent amount of distilled water instead. One hour after administration of PQ, rats of intervention group were treated with 5 ml / kg hydrogen saturated saline injected intra-peritoneally twice a day until the rats were sacrificed. The rats of poisoning group and control group were treated with intra-peritoneal injection of equivalent amount of normal saline. Arterial partial pressure of oxygen ( PaO2 ), 8-hydroxy-2' -desoxyguanosine (8-OHDG) and transforming growth factor β1 (TGF-β1) of lung tissue were measured on the 3rd and 21st day after PQ administration. Quantitative data was expressed as mean ±standard deviation (x-±s) . SPSS version 12. 0 package was applied for variance analysis and SNK-q test, and statistical differences were considered significant when P ＜ 0. 05. Results ① PaO2 decreased significantly in poisoning group (9. 34 ± 0. 47 kPa) and intervention group (10. 30±0.62 kPa) compared with control group (11.87 +/- 0.42 kPa) on the 3d (P ＜0.01), and as intervention group was compared with poisoning group, there was a significant difference ( P ＜ 0. 05 ) . On the 21st day, PaO2 was still lower in poisoning group (8. 36 ±0. 51 kPa) and intervention group ( 10. 14 ±0. 27 kPa) than that in control group ( 11.87 +0. 24 kPa) (P ＜0. 01 and P ＜0. 05, respectively), and as intervention group was compared with poisoning group, there was a significant difference ( P ＜ 0. 01 ) . ②The levels of 8-OHDG in lung tissue increased significantly in poisoning group (23.58±7. 18 ng/ml ) and intervention group (9. 49± 2. 45 ng/ml) on the 3rd day after PQ administration compared with control group (7.71 + 1.96 ng/ml) (P＜0. 01 and P＜0. 05, respectively), and as intervention group was compared with poisoning group there was a significant difference ( p ＜0. 01 ) . There were no significant differences in 8-OHDG level found among the groups on the 21st day after PQ administration (P ＞ 0. 05 ) . ③ The level of TGF-β1 (measured by mean optic density, MOD) in lung tissue of rats in poisoning group ( 10. 11±2.49 MOD) and intervention group (8. 14 + 1.58 MOD) exhibited in higher levels than control group (5.93 + 1. 98 MOD) on the 3rd and (5.97 + 2. 35 MOD) on the 21st day after PQ administration (P ＜0. 01 and P ＜0. 05, respectively), and however, a lower level of TGF-β1 was observed in intervention group on 3d and 21d compared with poisoning group (P ＜0. 05 and P ＜0. 01, respectively) . Conclusions Hydrogen saturated saline can alleviate oxidative stress, mitigate oxidative damage and inhibit pulmonary fibrosis of lung induced by PQ intoxication.