CAJ | 학술논문

目的探讨γδT细胞对肝癌细胞的杀伤作用.方法异戊烯焦磷酸(isopentenyl pyrophosphate,IPP)和rhIL-2活化正常人外周血单个核细胞(PBMC),用流式细胞仪分析活化后的γδT细胞含量,用ELISA法检测培养上清液中IFN-γ含量,用乳酸脱氢酶释放法测定活化γδT细胞的杀伤活性.结果体外活化的PBMC在10 d时γδT细胞比例从扩增前(4.34±1.79)%增加到(55.65±6.88)%,PBMC经IPP刺激培养后IFN-γ分泌量较不含IPP的对照组有显著增高,至第7天达峰值,活化后的淋巴细胞对肝癌细胞有较高杀伤活性.结论 IPP作为刺激剂可获得大量γδT细胞,刺激PBMC后可分泌大量IFN-γ,γδT细胞在体外对肝癌细胞有较高的杀伤活性.
목적 탐토γδT세포대간암세포적살상작용.방법 이무희초린산(isopentenyl pyrophosphate,IPP)화rhIL-2활화정상인외주혈단개핵세포(PBMC),용류식세포의분석활화후적γδT세포함량,용ELISA법검측배양상청액중IFN-γ함량,용유산탈경매석방법측정활화γδT세포적살상활성.결과 체외활화적PBMC재10 d시γδT세포비례종확증전(4.34±1.79)%증가도(55.65±6.88)%,PBMC경IPP자격배양후IFN-γ분비량교불함IPP적대조조유현저증고,지제7천체봉치,활화후적림파세포대간암세포유교고살상활성.결론 IPP작위자격제가획득대량γδT세포,자격PBMC후가분비대량IFN-γ,γδT세포재체외대간암세포유교고적살상활성.
Objective To investigate the cytotoxic effects of human γδT cells on hepatocellular carcinoma cells in vitro.Methods Normal human PBMC were activated by IPP and rhIL-2.The expansion of γδT cells were analyzed by flow cytometry.The concentration of interferon gamma in the supernatants was detected by ELISA.The cytotoxicity of activated lymphocytes against hepatocellular carcinoma cells were measured by the lactate dehydrogenase release assay in vitro.Results After PBMC activated by IPP and rhIL-2 for 10 days,the proportion of γδT cells were expanded from (4.34±1.79)% to (55.65±6.88 )%.The secretion of interferon gamma expanded significantly after PBMC stimulated by IPP,and reached its peak value at the seventh day.The secretion of interferon gamma is lower if it has no IPP.The activated lymphocytes stimulated by IPP were detected to have cytotoxicity in vitro against human hepatocellular carcinoma ceils.Conclusion The high proportion of γδT cells can be gained in human PBMC activated by IPP,PBMC stimulated by IPP can secret interferon gamma effectively and the activated γδT cells have high cytotoxlcity in vitro against human hepatocellular carcinoma cells.