CAJ | 학술논문

目的对上海市多家医院供水系统中分离的嗜肺军团菌血清1型(LP1)基因进行分型,探讨各家医院之间LP1基因型的相关性及其差别.方法 2009年5-9月份共采集上海市8家医院供水系统水样,分离LP1,应用脉冲场凝胶电泳技术进行LP1基因分型,对分型结果进行聚类分析,探讨菌株间的相互关系.结果上海市8家医院共采集193份水样,每份水量500 ml,5家医院的水样中共分离到24株LP1.24株LP1共分为A～E 6型,其中流行株为A(8株)、B(6株)及E(7株)3型.不同医院可存在相同的基因型,5家医院中3家存在不同的基因型.结论环境中的军团菌基因型较为稳定,主要的流行株为A、B和E型.由于一家医院内可存在多种基因型,故流行病学调查时需应用分子生物学技术确定感染源.
목적 대상해시다가의원공수계통중분리적기폐군단균혈청1형(LP1)기인진행분형,탐토각가의원지간LP1기인형적상관성급기차별.방법 2009년5-9월빈공채집상해시8가의원공수계통수양,분리LP1,응용맥충장응효전영기술진행LP1기인분형,대분형결과진행취류분석,탐토균주간적상호관계.결과 상해시8가의원공채집193빈수양,매빈수량500 ml,5가의원적수양중공분리도24주LP1.24주LP1공분위A～E 6형,기중류행주위A(8주)、B(6주)급E(7주)3형.불동의원가존재상동적기인형,5가의원중3가존재불동적기인형.결론 배경중적군단균기인형교위은정,주요적류행주위A、B화E형.유우일가의원내가존재다충기인형,고류행병학조사시수응용분자생물학기술학정감염원.
Objective To classify Legionella pneumophila serogroup 1( LP1 ) isolated from the water distribution systems in hospitals of Shanghai by using genotying methods, and therefore to explore the genetic relationship between different genotypes. Methods LP1 was isolated from 8 hospitals in Shanghai, and strains of LP1 were subtyped using pulse field gel electrophoresis ( PFGE ). Genotyping results of PFGE were classified as dendrogram, and cluster analysis was used to compare the genetic characteristics of different strains. Results Twenty-four strains of LP1 were isolated from 193 water specimens. LP1 belonged to 6PFGE genotypes, of which 21 belonged to 3 predominant genotypes. Different hospitals shared the same genotype, while some strains isolated from the same hospital had different genotypes. Conclusion The genetic characteristics are stable in environmental LP1. Molecular biology techniques should be added to epidemiological study for hospital acquired legionellosis due to the genetic diversity in one hospital.