CAJ | 학술논문

目的建立C57BL/6j小鼠人神经母细胞瘤移植瘤模型.方法取对数生长期SK-N-SH细胞1×107个/ml,分别接种3 d、7 d、3周、4周、5周、6周、7周C57BL/6j系小鼠及6周BALB/C系裸鼠,观察小鼠成瘤率、相对肿瘤体积(RTV)、相对肿瘤增长速率(K)、相对肿瘤倍增时间(Td)、宿主存活时间及移植瘤病理学情况.结果 C57BL/6j各年龄段及BALB/C持续成瘤率分别为:100%、100%、90%、70%、60%、0%、0%、100%.3周龄C57BL/6j与BALB/C比较RTV、K、Td及存活时间均差异无统计学意义(P＞0.05).结论在C57BL/6j小鼠体内成功建立人类异种移植瘤模型,3周龄为最适宜接种时间点.
목적 건립C57BL/6j소서인신경모세포류이식류모형.방법 취대수생장기SK-N-SH세포1×107개/ml,분별접충3 d、7 d、3주、4주、5주、6주、7주C57BL/6j계소서급6주BALB/C계라서,관찰소서성류솔、상대종류체적(RTV)、상대종류증장속솔(K)、상대종류배증시간(Td)、숙주존활시간급이식류병이학정황.결과 C57BL/6j각년령단급BALB/C지속성류솔분별위:100%、100%、90%、70%、60%、0%、0%、100%.3주령C57BL/6j여BALB/C비교RTV、K、Td급존활시간균차이무통계학의의(P＞0.05).결론 재C57BL/6j소서체내성공건립인류이충이식류모형,3주령위최괄의접충시간점.
Objective To investigate the feasibility of human neuroblastoma xenograft model in C57BL/6j mice. Methods Three-day, 7-day, 3-week, 4-week, 5-week, 6-week and 7-week age C57BL/6j and 6-week age BALB/C nude mice were subcutaneously inoculated with 1 × 107 cell/ml SK-NSH cells in logarithmic phase, respectively. The tumor generation rate, relative tumor volume ( RTV), relative growth rate (K), relative doubling time (Td), survival time and pathological changes were tested.Results The continuing tumor generation rate of C57BL/6j mice and BALB/C nude mice was 100% (10/10) at the 3rd day, 100% (10/10) at the 7th day, 90% (9/10) at the 3rd week and 70% (7/10) at the 4th week, 60%(6/10) at the 5th week, 0%(0/10) at the 6th week, 0%(0/10) at the 7th week, and 100% (10/10) at the 6th week, respectively. There was no significant difference in RTV, K, Td, and survival time (P＞0.05) between 3-week age C57 BL/6j mice and BALB/C nude mice. Conclusion Human neuroblastoma xenograft models were successfully established the in C57BL/6j mice, and 3-week age was the most appropriate opportunity of vaccination.