中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
5期
460-465
,共6页
赵雁林%王敬%张海青%易玲%李惠文%韦攀健%赵丹%王小珏%郑小芳%张洪涛
趙雁林%王敬%張海青%易玲%李惠文%韋攀健%趙丹%王小玨%鄭小芳%張洪濤
조안림%왕경%장해청%역령%리혜문%위반건%조단%왕소각%정소방%장홍도
天然调节性T细胞%CD4+CD25highFoxp3+%结核病%免疫病理
天然調節性T細胞%CD4+CD25highFoxp3+%結覈病%免疫病理
천연조절성T세포%CD4+CD25highFoxp3+%결핵병%면역병리
Natural regulatory T cell%CD4+CD25highFoxp3+%Tuberculosis%Immunopathology
目的 检测调节性T细胞(Tr)在结核病病人是否增高,确证Tr与结核免疫病理的关联性,为Tr功能研究提供疾病模型.方法 流式细胞术(FACS)分选CD3+CD4+T及CD3+CD8+T等主要淋巴细胞亚群,通过RT-PCR测定Foxp3基因在正常和结核病病人T细胞亚群中的表达,FACS多色分析Foxp3 T细胞在CD4+CD25+细胞群体中的分布,累积病例分析Tr细胞在外周血扩增情况,免疫组化方法分析Tr在结核病理的浸润,结合临床资料分析Tr数量的改变与结核病的关联性.结果 Foxp3基因在正常和结核病病人CD3+ CD4+T细胞中特异性扩增,FACS分析表明foxp3主要表达于CD4+ CD25high亚群中,占CD4+ CD25highT细胞的80%以上,以CD4+CD2high Foxp3+三联标志检测40例正常与51例活动性结核病病人外周血Tr占CD4+T细胞比例分别为(0.23±0.20)%和(1.01±1.00)%,结核病病人较正常人高4.4倍,Tr在结核病明显扩增(P<0.01),结核病理组织中有高频率Foxp3+细胞浸润.Tr扩增与结核病有效治疗和预后的关联性有深入探讨价值.结论 Tr细胞在结核病病人增高,并与结核免疫病理损伤相关,结核病可作为Tr细胞功能研究的疾病模型.
目的 檢測調節性T細胞(Tr)在結覈病病人是否增高,確證Tr與結覈免疫病理的關聯性,為Tr功能研究提供疾病模型.方法 流式細胞術(FACS)分選CD3+CD4+T及CD3+CD8+T等主要淋巴細胞亞群,通過RT-PCR測定Foxp3基因在正常和結覈病病人T細胞亞群中的錶達,FACS多色分析Foxp3 T細胞在CD4+CD25+細胞群體中的分佈,纍積病例分析Tr細胞在外週血擴增情況,免疫組化方法分析Tr在結覈病理的浸潤,結閤臨床資料分析Tr數量的改變與結覈病的關聯性.結果 Foxp3基因在正常和結覈病病人CD3+ CD4+T細胞中特異性擴增,FACS分析錶明foxp3主要錶達于CD4+ CD25high亞群中,佔CD4+ CD25highT細胞的80%以上,以CD4+CD2high Foxp3+三聯標誌檢測40例正常與51例活動性結覈病病人外週血Tr佔CD4+T細胞比例分彆為(0.23±0.20)%和(1.01±1.00)%,結覈病病人較正常人高4.4倍,Tr在結覈病明顯擴增(P<0.01),結覈病理組織中有高頻率Foxp3+細胞浸潤.Tr擴增與結覈病有效治療和預後的關聯性有深入探討價值.結論 Tr細胞在結覈病病人增高,併與結覈免疫病理損傷相關,結覈病可作為Tr細胞功能研究的疾病模型.
목적 검측조절성T세포(Tr)재결핵병병인시부증고,학증Tr여결핵면역병리적관련성,위Tr공능연구제공질병모형.방법 류식세포술(FACS)분선CD3+CD4+T급CD3+CD8+T등주요림파세포아군,통과RT-PCR측정Foxp3기인재정상화결핵병병인T세포아군중적표체,FACS다색분석Foxp3 T세포재CD4+CD25+세포군체중적분포,루적병례분석Tr세포재외주혈확증정황,면역조화방법분석Tr재결핵병리적침윤,결합림상자료분석Tr수량적개변여결핵병적관련성.결과 Foxp3기인재정상화결핵병병인CD3+ CD4+T세포중특이성확증,FACS분석표명foxp3주요표체우CD4+ CD25high아군중,점CD4+ CD25highT세포적80%이상,이CD4+CD2high Foxp3+삼련표지검측40례정상여51례활동성결핵병병인외주혈Tr점CD4+T세포비례분별위(0.23±0.20)%화(1.01±1.00)%,결핵병병인교정상인고4.4배,Tr재결핵병명현확증(P<0.01),결핵병리조직중유고빈솔Foxp3+세포침윤.Tr확증여결핵병유효치료화예후적관련성유심입탐토개치.결론 Tr세포재결핵병병인증고,병여결핵면역병리손상상관,결핵병가작위Tr세포공능연구적질병모형.
Objective To determine whether regulatory T cells(Tr)are increased in patients with tuberculosis and whether they are associated with its immunopathology.Meantime,to investigate the possibility of tuberculosis(TB)as a model for studying Tr functions.Methods The lymphocyte subsets were isolated from peripheral blood mononuclear cells by sorting with flow cytometry.Total cellular RNA was extracted and RT-PCR was performed to detect the Foxp3 mRNA in purified CD3+CIM+T cells,CD3+CD8+T cells and non-CD3+CD4+CD8+T cells.Using FACS analysis.we further investigated the distribution of Foxp3+ population in CD4+ CD25+T cells.Finally,we compared the percentage of CD4+CD25highFoxp3+T cells present in 51 active patients with tuberculosis and 40 uninfected healthy control subjects by FACS.The detection of Tr infiltration of Foxp3+ cells were performed with immunohistochemistry(IHC)method on tuberculosis pathological sections.Results Foxp3 was specific expressed in CD3+CD4+T cells,either in tuberculosis patients or healthy control subjects.Foxp3+ T cells took about 85%fraction of CD4+ CD25highpopulation.We used CD4+CD25high Foxp3+as a detective markers for Tr in the FACS analysis.The results showed that patients with active TB had a 4.4 fold higher percentage within the CD4+T cells in peripheral blood compared to healthy control group(modian,1.01%vs 0.23%,P<0.01).Much higher frequency of Tr were found along with T cells infiltration at the tuberculosis pathological tissues.A few individuals that we can followed indicated the expanded Tr was declined after curative treatment with operation.Conclusion Tr cells are increased in tuberculosis patients and closely correlate with its immunopathology.Tuberculosis should be a valuable model for Tr functional study.
