CAJ | 학술논문

目的:通过观察家蚕Bombyx mori吞噬细胞的微细结构,来确定拟绛色细胞是否也具有吞噬功能.方法:用荧光小球微量注射家蚕pnd pS品系的幼虫,经荧光染色剂丫啶橙和碘化丙啶染色循环血细胞后,在荧光显微镜下观察并扫描拍摄.结果:观察发现除颗粒细胞和浆血细胞外,一些原血球细胞(血干细胞)和拟绛色细胞(多酚氧化酶)也能吞噬荧光小球.在拟绛色细胞里还发现许多和颗粒细胞一样的能被丫啶橙染色的颗粒.尽管在小球细胞中没有发现被吞噬的荧光小球,但该类血球有比较多的能被丫啶橙染色的大颗粒,这表明它们可能是已经被吞噬的凋亡小体.结论:除颗粒细胞和浆血细胞外,一些原血球细胞和拟绛色细胞也能吞噬荧光小球.说明拟绛色细胞也具有吞噬功能.
목적:통과관찰가잠Bombyx mori탄서세포적미세결구,래학정의강색세포시부야구유탄서공능.방법:용형광소구미량주사가잠pnd pS품계적유충,경형광염색제아정등화전화병정염색순배혈세포후,재형광현미경하관찰병소묘박섭.결과:관찰발현제과립세포화장혈세포외,일사원혈구세포(혈간세포)화의강색세포(다분양화매)야능탄서형광소구.재의강색세포리환발현허다화과립세포일양적능피아정등염색적과립.진관재소구세포중몰유발현피탄서적형광소구,단해류혈구유비교다적능피아정등염색적대과립,저표명타문가능시이경피탄서적조망소체.결론:제과립세포화장혈세포외,일사원혈구세포화의강색세포야능탄서형광소구.설명의강색세포야구유탄서공능.
In order to observe the fine structure of phagocytotic hemocytes with an attention to make sure whether oenonytoids are also phagocytotic in the silkworm, Bombyx mori. Methods: Larvae of pnd pS strain of the silkworm were injected with fluorescent icrobeads for this experiment. Stained with acridine orange and propidium iodide, the phagocytotic hemocytes were serially scanned for fine structure by the Radiance 2000 confocal scanning laser microscope (Bio-Rad), and confocal fluorescent images were processed with the Lasersharp 2000 software. Results: the observation showed that besides granulocytes and plasmatocytes, some prohemocytes (stem hemocytes) and oenocytoids (prophenoloxidase producer) could also phagocytose fluorescent beads. Furthermore, inside some oenocytoids there were lots of acridine orange green granules that were similar to granules in granulocytes. Spherulocytes were not observed to have phagocytosed any fluorescent beads, but they had large acridine orange green granules. These granules were supposed to be apoptotic bodies which should be introduced by phagocytosis. Conclusion: Besides granulocytes and plasmatocytes, the circulating hemocytes oenocytoids are also phagocytotic in the silkworm.