CAJ | 학술논문

目的观察沉默Annexin Ⅱ(A2)基因对骨髓瘤RPMI8226细胞增殖影响.方法采用A2siRNA转染人骨髓瘤RPMI8226细胞后应用real-time PCR和流式细胞术鉴定干扰效果.MTT法检测A2siRNA对细胞增殖的作用.结果 A2siRNA组较阴性对照组细胞生长抑制率在24h、48h、72h分别为16.87%±3.97%,34.92%±4.15%,39.62%±5.67% vs-8.93%±9.67%,-5.98%±2.56%,3.85%±5.48%(P＜0.05),A2siRNA可对RPMI8226细胞增殖起抑制作用.结论 SiRNA沉默A2基因可抑制骨髓瘤RPMI8226细胞增殖.
목적 관찰침묵Annexin Ⅱ(A2)기인대골수류RPMI8226세포증식영향.방법 채용A2siRNA전염인골수류RPMI8226세포후응용real-time PCR화류식세포술감정간우효과.MTT법검측A2siRNA대세포증식적작용.결과 A2siRNA조교음성대조조세포생장억제솔재24h、48h、72h분별위16.87%±3.97%,34.92%±4.15%,39.62%±5.67% vs-8.93%±9.67%,-5.98%±2.56%,3.85%±5.48%(P＜0.05),A2siRNA가대RPMI8226세포증식기억제작용.결론 SiRNA침묵A2기인가억제골수류RPMI8226세포증식.