青岛大学医学院学报
青島大學醫學院學報
청도대학의학원학보
ACTA ACADEMIAE MEDICINAE QINGDAO UNIVERSITATIS
2009年
4期
368-370
,共3页
白细胞介素17%心脏移植%宿主抗移植物反应%早期诊断
白細胞介素17%心髒移植%宿主抗移植物反應%早期診斷
백세포개소17%심장이식%숙주항이식물반응%조기진단
Interleukin-17%Heart transplantation%Host versus graft reaction%Early diagnosis
目的 观察白细胞介素17(IL-17)在大鼠心脏移植急性排斥反应中含量的变化及其在排斥反应早期诊断中的作用.方法 采用改良心脏移植术式建立大鼠移植模型.实验组取15只SD大鼠的心脏移植于Wistar 大鼠,对照组取15 只Wistar大鼠的心脏移植于Wistar大鼠.实验组于术后第1、3天和发生急性排斥反应时处死大鼠取血和供体心脏,对照组于术后第1、3、8天获取标本(每次5只).应用酶联免疫吸附实验方法检测血清中IL-17的含量,心脏经过固定和染色后置显微镜下观察心肌组织.结果 实验组急性排斥反应平均发生时间为(7.8±2.9)d.实验组中术后第3天和排斥反应发生时血清IL-17含量明显高于对照组(t=-3.126、-11.043,P<0.05);排斥反应发生时IL-17的含量较术后第1天和第3天显著增高(F=15.113,q=3.72、5.21,P<0.05). 对照组术后第1、3、8天IL-17差异无统计学意义 (t=-0.307～0.097,P>0.05).实验组排斥反应发生时心脏排斥反应病理分级为 4 级,对照组为 0 级.结论 在大鼠心脏移植急性排斥反应发生前 5 d 血清 IL-17 水平已明显升高,排斥发生时达高峰,监测血清 IL-17 的含量可作为早期诊断急性排斥反应的一种方法.
目的 觀察白細胞介素17(IL-17)在大鼠心髒移植急性排斥反應中含量的變化及其在排斥反應早期診斷中的作用.方法 採用改良心髒移植術式建立大鼠移植模型.實驗組取15隻SD大鼠的心髒移植于Wistar 大鼠,對照組取15 隻Wistar大鼠的心髒移植于Wistar大鼠.實驗組于術後第1、3天和髮生急性排斥反應時處死大鼠取血和供體心髒,對照組于術後第1、3、8天穫取標本(每次5隻).應用酶聯免疫吸附實驗方法檢測血清中IL-17的含量,心髒經過固定和染色後置顯微鏡下觀察心肌組織.結果 實驗組急性排斥反應平均髮生時間為(7.8±2.9)d.實驗組中術後第3天和排斥反應髮生時血清IL-17含量明顯高于對照組(t=-3.126、-11.043,P<0.05);排斥反應髮生時IL-17的含量較術後第1天和第3天顯著增高(F=15.113,q=3.72、5.21,P<0.05). 對照組術後第1、3、8天IL-17差異無統計學意義 (t=-0.307～0.097,P>0.05).實驗組排斥反應髮生時心髒排斥反應病理分級為 4 級,對照組為 0 級.結論 在大鼠心髒移植急性排斥反應髮生前 5 d 血清 IL-17 水平已明顯升高,排斥髮生時達高峰,鑑測血清 IL-17 的含量可作為早期診斷急性排斥反應的一種方法.
목적 관찰백세포개소17(IL-17)재대서심장이식급성배척반응중함량적변화급기재배척반응조기진단중적작용.방법 채용개양심장이식술식건립대서이식모형.실험조취15지SD대서적심장이식우Wistar 대서,대조조취15 지Wistar대서적심장이식우Wistar대서.실험조우술후제1、3천화발생급성배척반응시처사대서취혈화공체심장,대조조우술후제1、3、8천획취표본(매차5지).응용매련면역흡부실험방법검측혈청중IL-17적함량,심장경과고정화염색후치현미경하관찰심기조직.결과 실험조급성배척반응평균발생시간위(7.8±2.9)d.실험조중술후제3천화배척반응발생시혈청IL-17함량명현고우대조조(t=-3.126、-11.043,P<0.05);배척반응발생시IL-17적함량교술후제1천화제3천현저증고(F=15.113,q=3.72、5.21,P<0.05). 대조조술후제1、3、8천IL-17차이무통계학의의 (t=-0.307～0.097,P>0.05).실험조배척반응발생시심장배척반응병리분급위 4 급,대조조위 0 급.결론 재대서심장이식급성배척반응발생전 5 d 혈청 IL-17 수평이명현승고,배척발생시체고봉,감측혈청 IL-17 적함량가작위조기진단급성배척반응적일충방법.
Objective To study the changes of serum interleukin-17 (IL-17) levels in acute rejection of heart transplantation in rat and their significance in early diagnosis of this reaction. Methods A heart-transplant model was established using a modified procedure. The experimental rats were divided into experiment group and control group. In the experiment, 15 hearts of Sprague-Dawley (SD) rat were transplanted to 15 Wistar rats. The rats in the experiment were sacrificed on day 1, 3 post-transplantation and when acute rejection occurred, the transplanted hearts were removed; in the control, the transplanted hearts were removed on day 1, 3 and 8, postoperatively, five hearts were collected each time in both groups. IL-17 was detected by enzyme-linked immunosorbent assay (ELISA) and the hearts were fixed and stained, the cardiac muscle was observed microscopically. Results The average rejection time in the experiment was 7.8±2.9 days, the level of IL-17 on the third day post-transplantation and during the rejection was significantly higher than that of the control (t=-3.126,-11.043;P<0.05), the IL-17 level at rejection was higher than that on the first and third day after procedure (F=15.113;q=3.72,5.21;P<0.05). In the control, no statistical difference of the IL-17 levels between the first, third and eighth day after transplantation was observed (t=-0.307-0.097,P>0.05). The pathologic grade was four in the experiment at rejection, compared with grade 0 for the control. Conclusion Serum IL-17 had already significantly increased five days before the episode of the rejection, reaching its peak at the attack, which suggests that IL-17 plays an important role in acute rejection. The detection of serum IL-17 might be used as a marker for an early diagnosis of this reaction.
