CAJ | 학술논문

为了筛选评价甘肃境内鼢鼠凸颅亚属母性遗传多样性分子标记,研究该亚属的分子系统发育.测定了分布于甘肃11个不同地方31只属于鼢鼠中凸颅亚属的不同鼢鼠,其中包括:9只高原鼢鼠、9只甘肃鼢鼠、9只斯氏鼢鼠、4只秦岭鼢鼠的细胞色素b基因全序列1 140 bp.从GenBank获得包括平颅亚属在内的21个细胞色素b全序列总共52条全序列,用MEGA软件分别绘制出本研究的31条全序列和总共52条全序列的2个系统发育树.运用测定序列,结合相关软件分析31条细胞色素b基因全序列的变化特征;分析和探讨本试验的Eospalax亚属4个种的分类及系统发育.本研究为鼢鼠的系统发育提供了分子理论依据.
위료사선평개감숙경내분서철로아속모성유전다양성분자표기,연구해아속적분자계통발육.측정료분포우감숙11개불동지방31지속우분서중철로아속적불동분서,기중포괄:9지고원분서、9지감숙분서、9지사씨분서、4지진령분서적세포색소b기인전서렬1 140 bp.종GenBank획득포괄평로아속재내적21개세포색소b전서렬총공52조전서렬,용MEGA연건분별회제출본연구적31조전서렬화총공52조전서렬적2개계통발육수.운용측정서렬,결합상관연건분석31조세포색소b기인전서렬적변화특정;분석화탐토본시험적Eospalax아속4개충적분류급계통발육.본연구위분서적계통발육제공료분자이론의거.
Maternity genetic diversity of the subgenus Eospalax in Gansu province was evaluated by studying the molecular phylogenesis of this subgenus. The nucleotide complete sequence variations of mitochondrial DNA (mtDNA) and cytochrome B gene (1 140 bp) were examined. Sequence data were obtained from 31 samples of the subgenus Eospalax from 11 different places in Gansu province and 21 other sequences were acquired from GenBank. Four Gansu species were Qinling zokor, Gansu zokor, Bailey's zokor, and Smith's zokor. Two phylogenetic trees were constructed, one from the 31 near complete Cytb sequences from our experiment and 52 Cyt which were aligned with GeneBank. Using these results and related software, we analysed the change of Cytb gene sequences and discussed the classification and phylogenesis of four species of the subgenus Eospalax. This study provides the molecular basis for the phylogenesis of the subgenus Eospalax.