CAJ | 학술논문

利用聚合酶链式反应技术(PCR)从拟南芥(Arabidopsis thaliana)植株中扩增出CBF1和CBF4基因,并将其连接到pGEM-T vector载体上.PCR和酶切鉴定后进行基因测序,结果表明所克隆的CBF1和CBF4基因序列与GenBank中基因序列同源性分别可达99.84%和99.41%,说明这两个片段确为拟南芥CBF1和CBF4基因.
이용취합매련식반응기술(PCR)종의남개(Arabidopsis thaliana)식주중확증출CBF1화CBF4기인,병장기련접도pGEM-T vector재체상.PCR화매절감정후진행기인측서,결과표명소극륭적CBF1화CBF4기인서렬여GenBank중기인서렬동원성분별가체99.84%화99.41%,설명저량개편단학위의남개CBF1화CBF4기인.
CBF1 and CBF4 genes were obtained by PCR technique from the plants of Arabidopsis thaliana and then connected those into the pGEM-T vector.PCR,Restriction enzyme digestion and Sequencing results showed that the cloned gene sequences of CBF1 and CBF4 have highly homologous with GenBank,reached 99.84%,99.41%Respectively.Thus,the two fragments were proved as CBF1 and CBF4 gene fragments