CAJ | 학술논문

目的:建立了一种同时检测莪术油中莪术醇和β-榄香烯的气相色谱分析方法.方法:以PEG弹性石英毛细管柱(30 m×0.32 mm)分离,氢火焰离子检测器(FID)检测,氮气为载气,采用程序升温法,即80℃保留7 min,以20℃/min的速度升温至140℃保留15 min,气化室温度为210℃,检测温度:200℃,柱前压为0.27 MPa,进样量为1 μL,采用外标法定量.结果:莪术醇在0.230 5～3.23 mg/mL范围内线性良好,r=0.999 3;平均回收率为100.4%,RSD为1.1%,β-榄香烯在0.237 5～3.33 mg/mL范围内线性良好,r=0.999 7;平均回收率为100.2%,RSD为2.2%.结论:本法具有简单、快速、准确、灵敏度高的优点,适用于成分复杂的莪术油体系中莪术醇和β-榄香烯的同时检测.
목적:건립료일충동시검측아술유중아술순화β-람향희적기상색보분석방법.방법:이PEG탄성석영모세관주(30 m×0.32 mm)분리,경화염리자검측기(FID)검측,담기위재기,채용정서승온법,즉80℃보류7 min,이20℃/min적속도승온지140℃보류15 min,기화실온도위210℃,검측온도:200℃,주전압위0.27 MPa,진양량위1 μL,채용외표법정량.결과:아술순재0.230 5～3.23 mg/mL범위내선성량호,r=0.999 3;평균회수솔위100.4%,RSD위1.1%,β-람향희재0.237 5～3.33 mg/mL범위내선성량호,r=0.999 7;평균회수솔위100.2%,RSD위2.2%.결론:본법구유간단、쾌속、준학、령민도고적우점,괄용우성분복잡적아술유체계중아술순화β-람향희적동시검측.
AIM:To develop a new method of gas chromatography for simultaneously determining curcumol contentin and β-elemene in Zedoary Turmeric Oil.METHODS:Separation and quantification were achieved on a quartz capillary column(30 m×0.32 mm)of PEG with a column temperature gradient from 80℃(holding for 7 min)to 140℃ at a rate of 20℃/min,holding for 15 min,taking N2 as loader.The temperature for gasification was 210℃;the temperature for detection was 200℃;the compressive stress of column was 0.27 MPa.RESULTS:Under the optimal chromatographic conditions,the linear range of curcumol contentin was within 0.230 5-3.23 mg/mL and the linear range of the β-elemene was within 0.237 5-3.33 mg/mL.The average recovery of curcumol contentin was 100.4% with a relative standard deviation(RSD)of 1.1% and β-elemene was 100.2% with a RSD of 2.2%.CONCLUSION:The results show that it is a simple,rapid,accurate and sensitive method which is suitable for analysis of complex component(curcumol contentin and β-elemene)in Zedoary Turmeric Oil at the same time.