CAJ | 학술논문

目的探讨肾素(renin,REN)基因G10631A和血管紧张素原(angiotensinogen,AGT)基因T704C单核苷酸多态性与脑梗死的关系,从分子水平探讨脑梗死发病的机制及特点.方法采用病例对照研究设计,通过聚合酶链反应-限制性片段长度多态性技术,检测82例脑梗死患者和89例对照者REN G10631A和AGT T704C多态性,比较病例组与对照组基因型和等位基因频率的差异.结果脑梗死组KEN 10631AA基因型频率(31.7%对10.1%,χ2=12.816,P=0.002)和A等位基因频率(49.4%对30.3%χ2=12.969,P=0.000)以及AGT 704 CC基因型频率(63.4%对34.8%,χ2=15.029,P=0.001)和C等位基因频率(79.9%对61.2%,χ2=14.173,P=0.000)均显著高于对照组;单倍型704C-1063lA频率也显著高于对照组(P=0.001).结论 REN 10631AA基因型和A等位基因以及AGT 704CC基因型和C等位基因可能为脑梗死的易感因素,单倍型704C-10631A可能是脑梗死发病的遗传危险因素.
목적 탐토신소(renin,REN)기인G10631A화혈관긴장소원(angiotensinogen,AGT)기인T704C단핵감산다태성여뇌경사적관계,종분자수평탐토뇌경사발병적궤제급특점.방법 채용병례대조연구설계,통과취합매련반응-한제성편단장도다태성기술,검측82례뇌경사환자화89례대조자REN G10631A화AGT T704C다태성,비교병례조여대조조기인형화등위기인빈솔적차이.결과 뇌경사조KEN 10631AA기인형빈솔(31.7%대10.1%,χ2=12.816,P=0.002)화A등위기인빈솔(49.4%대30.3%χ2=12.969,P=0.000)이급AGT 704 CC기인형빈솔(63.4%대34.8%,χ2=15.029,P=0.001)화C등위기인빈솔(79.9%대61.2%,χ2=14.173,P=0.000)균현저고우대조조;단배형704C-1063lA빈솔야현저고우대조조(P=0.001).결론 REN 10631AA기인형화A등위기인이급AGT 704CC기인형화C등위기인가능위뇌경사적역감인소,단배형704C-10631A가능시뇌경사발병적유전위험인소.
Objective To investigate the relationship between renin (REN) gene G10631A, angiotensinogen (AGT) gene T704C mononucleotide polymorphisms and cerebral infarction and to investigate the mechanisms and characteristics of cerebral infarction from molecular level. Methods REN gene G1063A and AGT gene T704C polymorphisms in 82 patients with cerebral infarction and 89 controls were detected with polymerase chain reactionrestriction fragment length polymorphism. The differences of the genotypes and allele frequencies were compared between the patient group and the control group. Results The frequency of REN 10631AA genotype (31. 7% vs. 10. 1%,χ2 =12. 816, P = 0. 002) and the frequency of A genotype (49. 4% vs. 30. 3% χ2 = 12. 969, P =0. 000), as well as the frequency of AGT 704 CC genotype (63. 4% vs. 34. 8% χ2 = 15. 029, P = 0. 001) and the frequency of A genotype (79. 9% vs. 61. 2% χ2 = 14. 173, P = 0. 000) in the cerebral infarction group were all significantly higher than those in the control group; the frequency of haplotype 704C 10631A was also significantly higher than that in the control group (P=0. 000). Conclusions REN 10631AA genetype and A allele as well as AGT 704 CC genetype and C allele may be the susceptible factors of cerebral infarction. Haplotype 704C-10631 A may be a genetic risk factor for the occurrence of cerebral infarction.