CAJ | 학술논문

目的探讨肾脏20-HETE下调在高脂饮食所致高血压发病机制的作用.方法以高脂饮食喂养3周龄的SD雄性大鼠至12周龄,监测正常饮食与高脂饮食两组大鼠的体质量与血压的变化,并以Western blot和HPLC等方法比较两组大鼠的的肾脏各部分20-HETE活性的差异.结果高脂饮食大鼠体质量和MBP在第12周龄明显较正常饮食组增高(414±7)vs(357±5)g,(132±8)vs(108±5)mm Hg,P<0.05),高脂饮食大鼠无论是全肾,还是肾实质各部分的20-HETE活性均明显下降,同时Western blot表明产生20-HETE的CYP4A表达也出现相应的变化.结论高脂饮食诱导的幼年型肥胖相关性高血压与肾实质的20-HETE下调有关.
목적 탐토신장20-HETE하조재고지음식소치고혈압발병궤제적작용.방법 이고지음식위양3주령적SD웅성대서지12주령,감측정상음식여고지음식량조대서적체질량여혈압적변화,병이Western blot화HPLC등방법비교량조대서적적신장각부분20-HETE활성적차이.결과 고지음식대서체질량화MBP재제12주령명현교정상음식조증고(414±7)vs(357±5)g,(132±8)vs(108±5)mm Hg,P<0.05),고지음식대서무론시전신,환시신실질각부분적20-HETE활성균명현하강,동시Western blot표명산생20-HETE적CYP4A표체야출현상응적변화.결론 고지음식유도적유년형비반상관성고혈압여신실질적20-HETE하조유관.
Objective To study renal's 20-hydroxyeicosatetraenoic acid (20-HETE) down-regulation in hypertension induced by high-fat diet. Methods SD male rats were fed with high fat diet from 3-weeks old to 12-weeks old ,compared the weight and MBP changes between high-fat diet and normal diet,analysed 20-HETE activity with RP HPLC and Western blot in different parts of kidney. Results Weight and MBP increased in high fat diet groups at the 12-weeks old(414±7) vs(357±5)g,(132±8)vs(108±5)ram Hg,P <0.05). There were significant down-regulation changes of 20-HETE activities between two groups, no matter in whole kidney or different part of renal parenchyma. And the same changes were found in CYP4A expression with western blot. Conclusion these results demonstrated that juvenile rats with obesity related hypertension induced by high-fat diet were related to the downregulation of 20-HETE in renal parenchyma.