中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2011年
1期
14-18
,共5页
袁钊辉%娄秉盛%林晓峰%张铁英%刘奕志
袁釗輝%婁秉盛%林曉峰%張鐵英%劉奕誌
원쇠휘%루병성%림효봉%장철영%류혁지
酵母多糖%眼内炎%眼感染,真菌性%白细胞介素1β%肿瘤坏死因子
酵母多糖%眼內炎%眼感染,真菌性%白細胞介素1β%腫瘤壞死因子
효모다당%안내염%안감염,진균성%백세포개소1β%종류배사인자
Zymosan%Endophthalmitis%Eye infections,fungal%Interleukin -1? Tumor necrosis factor-a
目的 观察真菌酵母多糖诱导的Wistar大鼠眼内炎模型的组织病理学特征、血眼屏障的改变和玻璃体内肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)的表达情况.方法 采用随机数字表法将大鼠分为生理盐水对照组(SC组,n=168)、眼内炎组(EO组,n=168).EO组玻璃体腔注射真菌酵母多糖溶液诱导眼内炎动物模型,SC组注入等量无菌生理盐水.注射后6、12 h及1、2、3、5、7 d,观察各组大鼠眼部炎性反应表现,并分别摘除眼球行组织病理学检查,同时取其房水检测蛋白质浓度.取玻璃体检测TNF-α、IL-1β水平.结果 注射后6 h～3 d,EO组眼内可见严重炎性反应,注射后7 d炎性反应基本消退.注射后1 d,EO组眼内白细胞浸润数量为(482.63±191.15)细胞/眼,达到高峰;注射后3 d浸润细胞数量迅速下降至(131.25±57.95)细胞/眼.注射后I d,EO组TNF-α和IL-1β浓度分别为(331.17±99.81)、(2 156.09±1 440.27)ng/L,均达到高峰并持续至注射后2 d,随后迅速下降;注射后7d,EO组TNF-α和IL-1β浓度分别为(5.55±5.27)、(43.66±18.73)ng/L.在各观察时点,均可观察到EO组房水蛋白质浓度较SC组注射后6 h显著增高(均P＜0.01).结论 真菌酵母多糖在Wistar大鼠可诱导出严重的实验性眼内炎,大量白细胞眼内浸润、血眼屏障破坏和玻璃体TNF-α、IL-1β的高水平表达是本实验性眼内炎模型的主要病理特点.
目的 觀察真菌酵母多糖誘導的Wistar大鼠眼內炎模型的組織病理學特徵、血眼屏障的改變和玻璃體內腫瘤壞死因子α(TNF-α)和白細胞介素1β(IL-1β)的錶達情況.方法 採用隨機數字錶法將大鼠分為生理鹽水對照組(SC組,n=168)、眼內炎組(EO組,n=168).EO組玻璃體腔註射真菌酵母多糖溶液誘導眼內炎動物模型,SC組註入等量無菌生理鹽水.註射後6、12 h及1、2、3、5、7 d,觀察各組大鼠眼部炎性反應錶現,併分彆摘除眼毬行組織病理學檢查,同時取其房水檢測蛋白質濃度.取玻璃體檢測TNF-α、IL-1β水平.結果 註射後6 h～3 d,EO組眼內可見嚴重炎性反應,註射後7 d炎性反應基本消退.註射後1 d,EO組眼內白細胞浸潤數量為(482.63±191.15)細胞/眼,達到高峰;註射後3 d浸潤細胞數量迅速下降至(131.25±57.95)細胞/眼.註射後I d,EO組TNF-α和IL-1β濃度分彆為(331.17±99.81)、(2 156.09±1 440.27)ng/L,均達到高峰併持續至註射後2 d,隨後迅速下降;註射後7d,EO組TNF-α和IL-1β濃度分彆為(5.55±5.27)、(43.66±18.73)ng/L.在各觀察時點,均可觀察到EO組房水蛋白質濃度較SC組註射後6 h顯著增高(均P＜0.01).結論 真菌酵母多糖在Wistar大鼠可誘導齣嚴重的實驗性眼內炎,大量白細胞眼內浸潤、血眼屏障破壞和玻璃體TNF-α、IL-1β的高水平錶達是本實驗性眼內炎模型的主要病理特點.
목적 관찰진균효모다당유도적Wistar대서안내염모형적조직병이학특정、혈안병장적개변화파리체내종류배사인자α(TNF-α)화백세포개소1β(IL-1β)적표체정황.방법 채용수궤수자표법장대서분위생리염수대조조(SC조,n=168)、안내염조(EO조,n=168).EO조파리체강주사진균효모다당용액유도안내염동물모형,SC조주입등량무균생리염수.주사후6、12 h급1、2、3、5、7 d,관찰각조대서안부염성반응표현,병분별적제안구행조직병이학검사,동시취기방수검측단백질농도.취파리체검측TNF-α、IL-1β수평.결과 주사후6 h～3 d,EO조안내가견엄중염성반응,주사후7 d염성반응기본소퇴.주사후1 d,EO조안내백세포침윤수량위(482.63±191.15)세포/안,체도고봉;주사후3 d침윤세포수량신속하강지(131.25±57.95)세포/안.주사후I d,EO조TNF-α화IL-1β농도분별위(331.17±99.81)、(2 156.09±1 440.27)ng/L,균체도고봉병지속지주사후2 d,수후신속하강;주사후7d,EO조TNF-α화IL-1β농도분별위(5.55±5.27)、(43.66±18.73)ng/L.재각관찰시점,균가관찰도EO조방수단백질농도교SC조주사후6 h현저증고(균P＜0.01).결론 진균효모다당재Wistar대서가유도출엄중적실험성안내염,대량백세포안내침윤、혈안병장파배화파리체TNF-α、IL-1β적고수평표체시본실험성안내염모형적주요병리특점.
Objective To investigate the histopathologic features, changes of blood-ocular barrier and the expression of tumor necrosis factor-α (TNF-β) and interleukin-1β(IL-1β in vitreous body of Wistar rats with zymosan-induced endophthalmitis.Methods Random number table method was used to assign the Wistar rats into saline control group (SC group,n=168) and endophthalmitis group (EO group,n=168).Rats of EO group received intravitreal injection of zymosan to induce endophthalmitis, while the control rats received injection of equivalent-ose sterile saline.The rat eyes were observed for signs of ocular inflammation and enucleated for histopathological examination at 6 h, 12 h, 1d, 2d, 3d, 5d and 7 d after injection respectively.Furthermore, aqueous humor for determination of protein levels and vitreous body for levels of TNF-α and IL-1βwere collected.Results Severe inflammatory response in the eyes was observed in EO group within 6 h to 3 d after zymosan injection, and was basically resolved after day 7.A peak intraocular leucocyte count was observed in EO group on day 1 [(482.63 ±91.15) cells/eye] that rapidly declined on day 3[(131.25±7.95) cells/eye].The increased levels of TNF-α and IL-1βin EO group peaked at 24 h after injection [TNF-α: (331.17±9.81) ng/L, IL-1β (2 156.09± 440.27) ng/L, respectively], persisted to 48 h after injection, and began to decline rapidly afterwards.On day 7 after injection, levels of TNF-α and IL-1βreturned to baseline[TNF-α: (5.55±2.27)ng/L, IL-1∞ (43.66±8.73) ng/L, respectively].Compared with SC group 6 h after injection, significantly higher protein levels in aqueous humor were detected in the EO group at all the time points (all P＜0.01).Conclusions Acute experimental endophthalmitis was successfully induced in the Wistar rats with intravitreal injection of zymosan.Massive leucocyte intraocular infiltration, blood-ocular barrier breakdown and high levels of TNF-α and IL-1βexpression in vitreous body may be the major pathologic characteristics in this experimental endophthalmitis model.
