中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2008年
7期
506-510
,共5页
郭宗铎%孙晓川%刘洪恩%郑履平
郭宗鐸%孫曉川%劉洪恩%鄭履平
곽종탁%손효천%류홍은%정리평
环孢素%白细胞介素-1β%轴索损伤
環孢素%白細胞介素-1β%軸索損傷
배포소%백세포개소-1β%축색손상
Cielesporin A%Intedeuldn-1β%Axonal injury
目的 观察大鼠轴索损伤后血清IL-1β的表达规律,以及环孢素A(CsA)对其表达的影响,探讨CsA的神经保护机制.方法 75只健康雄性SD大鼠随机分为3组:正常对照组(A组)5只、单纯视神经牵拉伤组(B组)35只、CsA处理组(C组)35只,B、C组又分为牵拉伤后或CsA治疗后1,3,6,12 h、1,3,7 d共7个观察时相点,不同时相点各5只大鼠.光镜下观察视网膜神经节细胞(RGCs)和轴索形态学变化,并采用放射免疫方法(平衡法)检测大鼠血清中IL-1β的含量.结果 (1)B组大鼠右侧视神经牵拉伤后3 d,RGCs明显稀疏;伤后7 d,神经纤维排列紊乱,分布稀疏.C组相应的病理变化明显减轻.(2)B组大鼠伤后3,6,12 h、1 d血清中IL-1β浓度明显高于A组,6 h达到高峰,随后逐渐下降,3 d后降至A组水平.C组大鼠血清IL-1β浓度变化规律与B组相似,3,6,12 h、l d血清中IL-1β浓度明显低于B组,但6,12 h、1 d仍高于A组.结论 IL-1β长时间过度表达可能参与了轴索损伤后的继发性病理变化;CsA可能通过减轻轴索损伤后的炎症反应对轴索损伤起保护作用.
目的 觀察大鼠軸索損傷後血清IL-1β的錶達規律,以及環孢素A(CsA)對其錶達的影響,探討CsA的神經保護機製.方法 75隻健康雄性SD大鼠隨機分為3組:正常對照組(A組)5隻、單純視神經牽拉傷組(B組)35隻、CsA處理組(C組)35隻,B、C組又分為牽拉傷後或CsA治療後1,3,6,12 h、1,3,7 d共7箇觀察時相點,不同時相點各5隻大鼠.光鏡下觀察視網膜神經節細胞(RGCs)和軸索形態學變化,併採用放射免疫方法(平衡法)檢測大鼠血清中IL-1β的含量.結果 (1)B組大鼠右側視神經牽拉傷後3 d,RGCs明顯稀疏;傷後7 d,神經纖維排列紊亂,分佈稀疏.C組相應的病理變化明顯減輕.(2)B組大鼠傷後3,6,12 h、1 d血清中IL-1β濃度明顯高于A組,6 h達到高峰,隨後逐漸下降,3 d後降至A組水平.C組大鼠血清IL-1β濃度變化規律與B組相似,3,6,12 h、l d血清中IL-1β濃度明顯低于B組,但6,12 h、1 d仍高于A組.結論 IL-1β長時間過度錶達可能參與瞭軸索損傷後的繼髮性病理變化;CsA可能通過減輕軸索損傷後的炎癥反應對軸索損傷起保護作用.
목적 관찰대서축색손상후혈청IL-1β적표체규률,이급배포소A(CsA)대기표체적영향,탐토CsA적신경보호궤제.방법 75지건강웅성SD대서수궤분위3조:정상대조조(A조)5지、단순시신경견랍상조(B조)35지、CsA처리조(C조)35지,B、C조우분위견랍상후혹CsA치료후1,3,6,12 h、1,3,7 d공7개관찰시상점,불동시상점각5지대서.광경하관찰시망막신경절세포(RGCs)화축색형태학변화,병채용방사면역방법(평형법)검측대서혈청중IL-1β적함량.결과 (1)B조대서우측시신경견랍상후3 d,RGCs명현희소;상후7 d,신경섬유배렬문란,분포희소.C조상응적병리변화명현감경.(2)B조대서상후3,6,12 h、1 d혈청중IL-1β농도명현고우A조,6 h체도고봉,수후축점하강,3 d후강지A조수평.C조대서혈청IL-1β농도변화규률여B조상사,3,6,12 h、l d혈청중IL-1β농도명현저우B조,단6,12 h、1 d잉고우A조.결론 IL-1β장시간과도표체가능삼여료축색손상후적계발성병리변화;CsA가능통과감경축색손상후적염증반응대축색손상기보호작용.
Objective To observe the expression of interleukin-1β (IL-1β) in blood serum after axonal injury in rats and investigate the effects of ciclosporin A (CsA) on it so as to discuss mechanism of CsA protecting neural function. Methods A total of 75 adult male SD rats were randomly divided into control group (Group A with 5 rats), only optic nerve stretch group (Group B with 35 rats) and stretch plus CsA treatment group (Group C with 35 rats). Stretch injury was induced in the right optic nerves of the rats in Group B and C. CsA at 20 mg/L was intraperitonealy injected in Group C immediately after stretch injury. Five animals from both Group B and Group C were killed at 1,3, 6, 12 hours and at days 1,3 and 7 after stretch injury or injection of CsA respectively. Morphological changes of optic nerves and retinal ganglion cells (RGCs) after stretch injury were examined under light microscope. In the mean- time, expression of IL-1β in the blood serum was observed by means of radioimmunoassay. Results (1) Histopathological observation showed lose of R GCs at day 3 and disarranged nerve fiber at day 7 after stretch injury of optic nerve in Group B, but significant amelioration of corresponding changes in Group C. (2) The expression of IL-1β in blood serum in Group B was significantly higher than that in Group A 3, 6, 12 hours and 1 day after injury. The expression of IL-1β reached peak at the 6th hour, then de- creased gradually and returned to the similar level of Group A after 3 days. The expression pattem of IL- 1 β in blood serum of Group C decreased more significantly at 3, 6, 12 hours and 1 day compared with that in Group B but was still higher than that of Group A at 6, 12 hours and 1 day. Conclusions The long-term and excessive expression of IL-1β may be involved in the secondary pathological changes after axonal injury. CsA exerts neuroprotective effect on injured axons mostly by attenuation of inflammation re- action after axonal injury.
