中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2010年
1期
52-57
,共6页
唐倩%陈建洪%吴坚%王萍%彭助力%梁焕友%陈晓峰%吴刚
唐倩%陳建洪%吳堅%王萍%彭助力%樑煥友%陳曉峰%吳剛
당천%진건홍%오견%왕평%팽조력%량환우%진효봉%오강
骨代用品%材料试验%骨缺损%生物活性玻璃%聚羟基丁酸-羟基戊酸共聚酯
骨代用品%材料試驗%骨缺損%生物活性玻璃%聚羥基丁痠-羥基戊痠共聚酯
골대용품%재료시험%골결손%생물활성파리%취간기정산-간기무산공취지
Bone substitutes%Materials testing%Bone defect%Bioactive glass%Poly hydroxybutyrate-hydroxyvalerate
目的 研究新型复合材料3-羟基丁酸-co-3-羟基戊酸共聚物-溶胶-凝胶生物活性玻璃(PHBV-SGBG)对骨髓基质细胞的细胞相容性,并建立动物骨缺损模型,探讨PHBV-SGBG的体内成骨效能.方法 采用MTT法和直接接触法检测复合材料PHBV-SGBG的细胞相容性.制备犬胫骨骨缺损模型,实验组在骨缺损区植入PHBV-SGBG材料,对照组不作处理,分别于术后2、4、8、12周取材,扫描电镜和X线能谱分析观察该材料的体内成骨效能.结果 复合材料PHBV-SGBG的细胞毒性分级在0～1之间,骨髓基质细胞可紧密附着于材料表面,黏附、生长、增殖,并有突起向材料的连通微孔内伸展.实验组术后2周已有明显新骨生成,术后4周时Ca/P元素比值(1.086±0.034),对照组为(0.793±0.053),术后12周时骨缺损区充填新生的成熟骨组织,扫描电镜和能谱分析显示实验组Ca峰值逐渐升高:12周时Ca/P元素比值(1.603±0.067),优于对照组(11456±0.036)(P<0.05).结论 复合材料PHBV-SGBG无细胞毒性,具有很好的骨传导性和骨再生能力,有望成为新型的骨组织工程材料.
目的 研究新型複閤材料3-羥基丁痠-co-3-羥基戊痠共聚物-溶膠-凝膠生物活性玻璃(PHBV-SGBG)對骨髓基質細胞的細胞相容性,併建立動物骨缺損模型,探討PHBV-SGBG的體內成骨效能.方法 採用MTT法和直接接觸法檢測複閤材料PHBV-SGBG的細胞相容性.製備犬脛骨骨缺損模型,實驗組在骨缺損區植入PHBV-SGBG材料,對照組不作處理,分彆于術後2、4、8、12週取材,掃描電鏡和X線能譜分析觀察該材料的體內成骨效能.結果 複閤材料PHBV-SGBG的細胞毒性分級在0～1之間,骨髓基質細胞可緊密附著于材料錶麵,黏附、生長、增殖,併有突起嚮材料的連通微孔內伸展.實驗組術後2週已有明顯新骨生成,術後4週時Ca/P元素比值(1.086±0.034),對照組為(0.793±0.053),術後12週時骨缺損區充填新生的成熟骨組織,掃描電鏡和能譜分析顯示實驗組Ca峰值逐漸升高:12週時Ca/P元素比值(1.603±0.067),優于對照組(11456±0.036)(P<0.05).結論 複閤材料PHBV-SGBG無細胞毒性,具有很好的骨傳導性和骨再生能力,有望成為新型的骨組織工程材料.
목적 연구신형복합재료3-간기정산-co-3-간기무산공취물-용효-응효생물활성파리(PHBV-SGBG)대골수기질세포적세포상용성,병건립동물골결손모형,탐토PHBV-SGBG적체내성골효능.방법 채용MTT법화직접접촉법검측복합재료PHBV-SGBG적세포상용성.제비견경골골결손모형,실험조재골결손구식입PHBV-SGBG재료,대조조불작처리,분별우술후2、4、8、12주취재,소묘전경화X선능보분석관찰해재료적체내성골효능.결과 복합재료PHBV-SGBG적세포독성분급재0～1지간,골수기질세포가긴밀부착우재료표면,점부、생장、증식,병유돌기향재료적련통미공내신전.실험조술후2주이유명현신골생성,술후4주시Ca/P원소비치(1.086±0.034),대조조위(0.793±0.053),술후12주시골결손구충전신생적성숙골조직,소묘전경화능보분석현시실험조Ca봉치축점승고:12주시Ca/P원소비치(1.603±0.067),우우대조조(11456±0.036)(P<0.05).결론 복합재료PHBV-SGBG무세포독성,구유흔호적골전도성화골재생능력,유망성위신형적골조직공정재료.
Objective To study the cytocompatibility of poly (3-hydroxybutyrate-co-3 -hydroxyvalerate)/sol-gel bioactive glass (PHBV/SGBG)composite with bone marrow stromal cells in vitro, and the in vivo osteogenesis efficiency of PHBV/SGBG composite under periosteal flap in bone defect animal models. Methods The cytocompatibility of PHBV/SGBG composite was tested by MTT assay and direct contact assay. Of established tibial defect canine models, PHBV/SGBG composite was embedded into the defects of the experimental group, while no treatment was given to the control group. Samples were harvested at weeks 2,4, 8, and 12 post-surgery. The effect of osteogenesis was inspected by scanning electron microscope observation and energy-dispersive X-ray analysis. Results The cytotoxicity score of PHBV/SGBG composite in the serial dilution extract and different culture time ranked from grades 0 to 1. Marrow stem cells (MSCs) attached to and then adhered firmly on the surface of PHBV/SGBG composite, and proliferated rapidly, with obvious cell prominences stretching into the micro-pores structure. In the experimental group, formation of new bone islands was observed at week 2 post-surgery, and mature bone regeneration at week 12. Scanning electron microscopy and energy-dispersive X-ray analysis showed increasing calcium peak in the experiment group. At week 4 Ca/P ratio was 1.086±0.034 in experiment group and 0.793±0.053 in control group. At week 12 Ca/P ratio was 1.603±0.067 in experiment group and 1.456±0.036 in control group (P<0.05). Conclusions PHBV/SGBG shows no cytotoxicity to MSCs, and possesses good osteo-conductibility and regeneration capability. It may be a promising composite for bone tissue engineering.
