中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
1期
47-50
,共4页
耿家宝%付红伟%王玲%王晓娟%关建民%常义宾%李凌君%朱永红%庄辉%刘全红%彭兴春
耿傢寶%付紅偉%王玲%王曉娟%關建民%常義賓%李凌君%硃永紅%莊輝%劉全紅%彭興春
경가보%부홍위%왕령%왕효연%관건민%상의빈%리릉군%주영홍%장휘%류전홍%팽흥춘
戊型肝炎病毒%抗-HEV%基因型%人兽共患病
戊型肝炎病毒%抗-HEV%基因型%人獸共患病
무형간염병독%항-HEV%기인형%인수공환병
Hepatitis E virus%Anti-HEV antibody%Genotype%Zoonosis
目的 调查北京地区与人密切接触的猪、牛、羊、马、驴、犬、鸡、鸭8种动物血清中戊型肝炎病毒(HEV)抗体流行率及该地区猪HEV基因型和亚型.方法 收集8种动物的血清标本及幼猪粪便标本,用双抗原夹心ELISA法检测血清中抗-HEV;用巢式反转录聚合酶链反应法(RT-nPCR)检测HEV RNA;对部分PCR产物进行克隆和测序,并对测序结果进行基因分型.结果 在8种动物中抗-HEV阳性率分别为猪80.43%(481/598),其中成猪(>6月龄)为87.86%(369/420),幼猪(2～3月龄)为62.92%(112/178);牛15.02%(52/346);马14.29%(40/280);驴0(0/26);羊9.88%(33/334);犬0(0/21);鸭3.03%(7/231);鸡2.53%(8/316).RT-nPCR检测3月龄以下猪粪便标本HEVRNA阳性率为66.67%(74/111).HEV RNA阳性的标本测序后可归为6株(分别命名为bjsw1、bjsw2、bjsw3、bjsw4、bjsw5和bjsw6),6株HEV开放读码框2(ORF2)部分核苷酸序列的相似性为94.5%～99.6%,与1、2、3、4型HEV的相似性分别为75.6%～78.6%、75.6%～76.2%、77.1%～80.7%和83.7%～94.5%.6株HEV与人HEV 4d亚型的同源性最高,为90.0%～94.5%.结论 北京市郊区猪、牛、马、羊、鸭、鸡中均存在HEV感染,其中猪抗-HEV流行率最高,驴、犬抗-HEV的流行率最低;6株猪HEV属于基因4型、4d亚型.
目的 調查北京地區與人密切接觸的豬、牛、羊、馬、驢、犬、鷄、鴨8種動物血清中戊型肝炎病毒(HEV)抗體流行率及該地區豬HEV基因型和亞型.方法 收集8種動物的血清標本及幼豬糞便標本,用雙抗原夾心ELISA法檢測血清中抗-HEV;用巢式反轉錄聚閤酶鏈反應法(RT-nPCR)檢測HEV RNA;對部分PCR產物進行剋隆和測序,併對測序結果進行基因分型.結果 在8種動物中抗-HEV暘性率分彆為豬80.43%(481/598),其中成豬(>6月齡)為87.86%(369/420),幼豬(2～3月齡)為62.92%(112/178);牛15.02%(52/346);馬14.29%(40/280);驢0(0/26);羊9.88%(33/334);犬0(0/21);鴨3.03%(7/231);鷄2.53%(8/316).RT-nPCR檢測3月齡以下豬糞便標本HEVRNA暘性率為66.67%(74/111).HEV RNA暘性的標本測序後可歸為6株(分彆命名為bjsw1、bjsw2、bjsw3、bjsw4、bjsw5和bjsw6),6株HEV開放讀碼框2(ORF2)部分覈苷痠序列的相似性為94.5%～99.6%,與1、2、3、4型HEV的相似性分彆為75.6%～78.6%、75.6%～76.2%、77.1%～80.7%和83.7%～94.5%.6株HEV與人HEV 4d亞型的同源性最高,為90.0%～94.5%.結論 北京市郊區豬、牛、馬、羊、鴨、鷄中均存在HEV感染,其中豬抗-HEV流行率最高,驢、犬抗-HEV的流行率最低;6株豬HEV屬于基因4型、4d亞型.
목적 조사북경지구여인밀절접촉적저、우、양、마、려、견、계、압8충동물혈청중무형간염병독(HEV)항체류행솔급해지구저HEV기인형화아형.방법 수집8충동물적혈청표본급유저분편표본,용쌍항원협심ELISA법검측혈청중항-HEV;용소식반전록취합매련반응법(RT-nPCR)검측HEV RNA;대부분PCR산물진행극륭화측서,병대측서결과진행기인분형.결과 재8충동물중항-HEV양성솔분별위저80.43%(481/598),기중성저(>6월령)위87.86%(369/420),유저(2～3월령)위62.92%(112/178);우15.02%(52/346);마14.29%(40/280);려0(0/26);양9.88%(33/334);견0(0/21);압3.03%(7/231);계2.53%(8/316).RT-nPCR검측3월령이하저분편표본HEVRNA양성솔위66.67%(74/111).HEV RNA양성적표본측서후가귀위6주(분별명명위bjsw1、bjsw2、bjsw3、bjsw4、bjsw5화bjsw6),6주HEV개방독마광2(ORF2)부분핵감산서렬적상사성위94.5%～99.6%,여1、2、3、4형HEV적상사성분별위75.6%～78.6%、75.6%～76.2%、77.1%～80.7%화83.7%～94.5%.6주HEV여인HEV 4d아형적동원성최고,위90.0%～94.5%.결론 북경시교구저、우、마、양、압、계중균존재HEV감염,기중저항-HEV류행솔최고,려、견항-HEV적류행솔최저;6주저HEV속우기인4형、4d아형.
Objective To investigate the prevalence of anti-hepatitis E virus (HEV) and genotypes of hepatitis E virus in 8 species of animals including swine, cattle, sheep, horse, donkey, dog, chicken and duck in the suburb of Beijing. Methods Serum samples were collected from the 8 species of animals, and fecal samples of younger swine were collected from 2 stock farms. Anti-HEV was detected by Double Antigen Sandwich Assay. HEV RNA from fecal samples was detected by a reverse transcription nested polymerase chain reaction (RT-nPCR). Parts of the PCR products were cloned and sequenced. The swine HEV sequences were analyzed genetically. Results The positive rates of anti-HEV in serum specimens of swine, cattle, horse, donkey, sheep, dog, duck and chicken were 80.43%(481/598), 15.02%(52/346), 14.29%(40/280) ,0(0/26) ,9.88%(33/334), 0(0/ 21) ,3.03% (7/231) and 2.53%(8/316), respectively. The anti-HEV prevalence of adult swine(≥6 months)and younger swine(≤3 months)were 87.86%(369/420)and 62.92%(112/178)respectively. 74 of 111 (66.67% ) pig faces were positive for HEV RNA. Sequence analysis on these positive samples showed that there were 6 groups of HEV designated as bjsw1, bjsw2, bjsw3, bjsw4, bjsw5 and bjsw6. The 6 strains of HEV shared 94.5%-99.6% sequence identity of partial HEV ORF2 nucleotide with each other. The identities of HEV ORF2 nucleotide sequences between the 6 strains and genotype 1, 2, 3 and 4 were 75.6%-78.6% , 75.6%-76.2%, 77.1%-80.7% and 83.7%-94.5%, respectively. The sequence identity between the 6 strains and human HEV genotype 4d was 90.0%-94.5% . Conclusion HEV infection was seen in swine, cattle, horse, sheep, duck and chicken in the suburbs of Beijing. The anti-HEV positive rate appeared the highest in swine and the lowest in dog and donkey. The six strains of HEV isolated from younger swine belonged to genotype 4d.
