中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
11期
1964-1966
,共3页
裴保安%段姗姗%吴立生%张存华%陈允震
裴保安%段姍姍%吳立生%張存華%陳允震
배보안%단산산%오립생%장존화%진윤진
坐骨神经损伤%电刺激%脊髓%运动神经元%背根神经节
坐骨神經損傷%電刺激%脊髓%運動神經元%揹根神經節
좌골신경손상%전자격%척수%운동신경원%배근신경절
Sciatic nerve injury%Electrical stimulus%Spinal cord%Motoneuron%Dorsalroot ganglion
目的 观察脊髓电刺激对大鼠坐骨神经切断后背根神经节(DRG)神经元和脊髓神经元的影响.方法 建立大鼠坐骨神经损伤模型,随机分为实验组(n=15)和对照组(n=15)；实验组行脊髓电刺激,对照组行假治疗.观察不同时期两组大鼠DRG神经元和脊髓神经元计数和超微结构；测定再生神经纤维髓鞘厚度及传导速度(NCV).结果 术后1、4、8周,实验组DRG神经元计数为15.74±1.47、14.17±1.20、13.33±0.47；对照组为10.99±1.38、10.33±0.78、9.20±0.56两者差异有统计学意义(P＜0.05).实验组脊髓神经元计数为17.16±0.50、15.86±1.27、11.55±0.95;对照组为12.16±0.92、10.27±0.44、9.24±0.78,两者差异有统计学意义(P＜0.05).结论 脊髓电刺激对周围神经损伤后DRG神经元和脊髓神经元具有保护作用.
目的 觀察脊髓電刺激對大鼠坐骨神經切斷後揹根神經節(DRG)神經元和脊髓神經元的影響.方法 建立大鼠坐骨神經損傷模型,隨機分為實驗組(n=15)和對照組(n=15)；實驗組行脊髓電刺激,對照組行假治療.觀察不同時期兩組大鼠DRG神經元和脊髓神經元計數和超微結構；測定再生神經纖維髓鞘厚度及傳導速度(NCV).結果 術後1、4、8週,實驗組DRG神經元計數為15.74±1.47、14.17±1.20、13.33±0.47；對照組為10.99±1.38、10.33±0.78、9.20±0.56兩者差異有統計學意義(P＜0.05).實驗組脊髓神經元計數為17.16±0.50、15.86±1.27、11.55±0.95;對照組為12.16±0.92、10.27±0.44、9.24±0.78,兩者差異有統計學意義(P＜0.05).結論 脊髓電刺激對週圍神經損傷後DRG神經元和脊髓神經元具有保護作用.
목적 관찰척수전자격대대서좌골신경절단후배근신경절(DRG)신경원화척수신경원적영향.방법 건립대서좌골신경손상모형,수궤분위실험조(n=15)화대조조(n=15)；실험조행척수전자격,대조조행가치료.관찰불동시기량조대서DRG신경원화척수신경원계수화초미결구；측정재생신경섬유수초후도급전도속도(NCV).결과 술후1、4、8주,실험조DRG신경원계수위15.74±1.47、14.17±1.20、13.33±0.47；대조조위10.99±1.38、10.33±0.78、9.20±0.56량자차이유통계학의의(P＜0.05).실험조척수신경원계수위17.16±0.50、15.86±1.27、11.55±0.95;대조조위12.16±0.92、10.27±0.44、9.24±0.78,량자차이유통계학의의(P＜0.05).결론 척수전자격대주위신경손상후DRG신경원화척수신경원구유보호작용.
Objective To observe the effect of epidural electrical stimulation on spinal cord motoneuron and dorsal root ganglion after sciatic nerve transection.Methods The model of sciatic nerve injury were established,and the rats were divided into 2 groups randomly.In the experimental group,the epidural stimulator was inserted into the correspond segments of spinal cord.In the control group,the same stimulator was inserted but no output current was given.The number of spinal cord motoneurons and dorsal root ganglion neurons was counted,and their ultrastructures were observed.The thickness of the myelin sheat of the regenerated nerve fiber and its conduction velocity at properly time were measured.Results At 1st,4th and 8th week after operation,the number of neurons of dorsal root ganglion in experimental group was 15.74 ± 1.47,14.17 ± 1.20 and 13.33 ±0.47,and that in control group was 10.99 ± 1.38,10.33 ±0.78 and 9.20 ±0.56 respectively.The number of neurons of anterior horn of spinal cord in experimental group was 17.16 ± 0.50,15.86 ± 1.27 and 11.55 ± 0.95,and that in control group was 12.16 ±0.92,10.27 ± 0.44 and 9.24 ± 0.78 respectively.Statistically,there was significant difference in the number of neurons between experimental group and control group (P ＜ 0.05 ).Conclusion Epidural pulse-electrical stimulation could prevent the apoptosis of perikaryon and protect the normal structures of the spinal cord motoneurons and dorsal root ganglions.
