中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2011年
7期
442-445
,共4页
胰腺肿瘤%补体C3%补体C4B1%载脂蛋白E类%肿瘤分期%淋巴转移
胰腺腫瘤%補體C3%補體C4B1%載脂蛋白E類%腫瘤分期%淋巴轉移
이선종류%보체C3%보체C4B1%재지단백E류%종류분기%림파전이
Pancreatic neoplasms%Complement C3%Complement C4B1%Apolipoproteins E%Neoplasm staging%Lymphatic metastasis
目的 探讨补体3(C3)、补体4B1(C4B1)、载脂蛋白(Apo)E在胰腺癌中的表达及其与TNM分期和胰腺癌淋巴转移的相关性.方法 收集胰腺癌病理切片标本38例及胰腺癌新鲜标本和癌旁正常胰腺组织各20例.分别应用免疫组织化学和Western印迹方法检测胰腺癌和正常胰腺组织中C3、C4B1、ApoE的表达情况,观察C3、C4B1、ApoE阳性表达率和灰度比的差异.使用SPSS 13.0统计软件包分析其与胰腺癌TNM分期和淋巴转移的相关性.结果 38例胰腺癌组织中C3、C4B1、ApoE表达率分别为73.68%(28/38)、86.84%(29/38)、76.31%(33/38),高于正常胰腺组织的42.11%(16/38)、26.32%(10/38)、42.11%(16/38),差异均有统计学意义(χ2分别＝7.77、19.01、16.60,P值分别=0.01、0.00、0.00).C3、C4B1、ApoE在胰腺癌中的灰度比分别为1.63±0.28、1.25±0.18、2.57±0.22,高于正常胰腺(0.88±0.19、0.65±0.13、1.28±0.24),差异均有统计学意义(t值分别=9.93、11.81、17.71,P值均=0.00).C3与胰腺癌的TNM分期和淋巴转移无关.C4B1、ApoE与TNM分期和淋巴转移密切相关.Ⅱ至Ⅳ期胰腺癌和有淋巴转移胰腺癌中C4B1、ApoE表达明显高于Ⅰ期胰腺癌和无淋巴转移胰腺癌.结论 C3、C4B1、ApoE在胰腺癌中均呈高表达.C3只参与胰腺癌的早期过程,与胰腺癌发展进程无关.C4B1、ApoE参与胰腺癌生长和转移.
目的 探討補體3(C3)、補體4B1(C4B1)、載脂蛋白(Apo)E在胰腺癌中的錶達及其與TNM分期和胰腺癌淋巴轉移的相關性.方法 收集胰腺癌病理切片標本38例及胰腺癌新鮮標本和癌徬正常胰腺組織各20例.分彆應用免疫組織化學和Western印跡方法檢測胰腺癌和正常胰腺組織中C3、C4B1、ApoE的錶達情況,觀察C3、C4B1、ApoE暘性錶達率和灰度比的差異.使用SPSS 13.0統計軟件包分析其與胰腺癌TNM分期和淋巴轉移的相關性.結果 38例胰腺癌組織中C3、C4B1、ApoE錶達率分彆為73.68%(28/38)、86.84%(29/38)、76.31%(33/38),高于正常胰腺組織的42.11%(16/38)、26.32%(10/38)、42.11%(16/38),差異均有統計學意義(χ2分彆＝7.77、19.01、16.60,P值分彆=0.01、0.00、0.00).C3、C4B1、ApoE在胰腺癌中的灰度比分彆為1.63±0.28、1.25±0.18、2.57±0.22,高于正常胰腺(0.88±0.19、0.65±0.13、1.28±0.24),差異均有統計學意義(t值分彆=9.93、11.81、17.71,P值均=0.00).C3與胰腺癌的TNM分期和淋巴轉移無關.C4B1、ApoE與TNM分期和淋巴轉移密切相關.Ⅱ至Ⅳ期胰腺癌和有淋巴轉移胰腺癌中C4B1、ApoE錶達明顯高于Ⅰ期胰腺癌和無淋巴轉移胰腺癌.結論 C3、C4B1、ApoE在胰腺癌中均呈高錶達.C3隻參與胰腺癌的早期過程,與胰腺癌髮展進程無關.C4B1、ApoE參與胰腺癌生長和轉移.
목적 탐토보체3(C3)、보체4B1(C4B1)、재지단백(Apo)E재이선암중적표체급기여TNM분기화이선암림파전이적상관성.방법 수집이선암병리절편표본38례급이선암신선표본화암방정상이선조직각20례.분별응용면역조직화학화Western인적방법검측이선암화정상이선조직중C3、C4B1、ApoE적표체정황,관찰C3、C4B1、ApoE양성표체솔화회도비적차이.사용SPSS 13.0통계연건포분석기여이선암TNM분기화림파전이적상관성.결과 38례이선암조직중C3、C4B1、ApoE표체솔분별위73.68%(28/38)、86.84%(29/38)、76.31%(33/38),고우정상이선조직적42.11%(16/38)、26.32%(10/38)、42.11%(16/38),차이균유통계학의의(χ2분별＝7.77、19.01、16.60,P치분별=0.01、0.00、0.00).C3、C4B1、ApoE재이선암중적회도비분별위1.63±0.28、1.25±0.18、2.57±0.22,고우정상이선(0.88±0.19、0.65±0.13、1.28±0.24),차이균유통계학의의(t치분별=9.93、11.81、17.71,P치균=0.00).C3여이선암적TNM분기화림파전이무관.C4B1、ApoE여TNM분기화림파전이밀절상관.Ⅱ지Ⅳ기이선암화유림파전이이선암중C4B1、ApoE표체명현고우Ⅰ기이선암화무림파전이이선암.결론 C3、C4B1、ApoE재이선암중균정고표체.C3지삼여이선암적조기과정,여이선암발전진정무관.C4B1、ApoE삼여이선암생장화전이.
Objective To investigate the expressions of complements 3 (C3), 4B1 (C4B1) and apolipoprotein E (ApoE) in pancreatic cancer and relations with TNM staging and lymph node metastasis of pancreatic cancer. Methods Thirty-eight pancreatic cancer biopsy specimens, 20 fresh pancreatic cancer specimens and 20 adjacent normal tissues of pancreatic cancer were collected. The expressions of C3, C4B1, ApoE in pancreatic cancers and normal pancreatic tissues were detected by immunohistochemistry and Western-Blot, the positive expression rates of C3, C4B1, ApoE and the differences in gray scale were also observed. Their association with pancreatic cancer TNM staging and lymph node metastasis were analyzed by SPSS 13.0. Results The expression rates of C3, C4B1, ApoE in pancreatic cancer were 73.68% (28/38), 86.84%(29/38) and 76.31% (33/38) respectively, higher than those in normal pancreatic tissues which were 42.11% (16/38), 26.32% (10/38) and 42.11% (16/38) accordingly, the differences were statistically significant (χ2 was 7.77, 19.01, 16.6, and P value were 0.01, 0.00, 0.00 respectively). The gray scale of C3, C4B1 and ApoE in pancreatic cancer were 1.63±0.28,1.25±0.18 and 2.57±0.22 respectively, higher than those in normal pancreatic tissue (0.88±0.19,0.65±0.13,1.28±0.24 respectively), the differences were statistically significant (t value were 9.93,11.81,17.71 and all P value were 0.00, respectively). There was no association between C3 and TNM staging or lymphatic metastasis of pancreatic cancer. C4B1 and ApoE were closely related with TNM stage and lymph node metastases. The expressions of C4B1 and ApoE in stage Ⅱ to Ⅳ pancreatic cancer or with lymphatic metastasis were significantly higher than those in stage Ⅰpancreatic cancer and those without lymph node metastasis. Conclusion C3, C4B1 and ApoE were all highly expressed in pancreatic cancer. C3 was only involved in early event in pancreatic cancer, not related with development of pancreatic cancer. C4B1 and ApoE were involved in tumor growth and metastasis.
