CAJ | 학술논문

目的探讨Ca2+-非依赖性磷酸脂酶A2(iPLA2)在人胰岛的表达及在胰岛素分泌功能中的作用.方法正常人胰腺组织切片免疫组织化学染色及人胰岛Western印迹方法检测,观察iPLA2在人胰岛中的表达情况;随机分组对照研究iPLA2选择性抑制剂溴烯醇内酯(BEL)对离体人胰岛的葡萄糖刺激引起胰岛素分泌反应的影响.结果在人胰岛iPLA2高表达,与抗胰岛素染色分布一致,而外分泌腺很少表达;与对照组相比,BEL处理组胰岛素分泌反应明显减弱(P＜0.01),BEL通过抑制iPLA2活性抑制了葡萄糖刺激引起的离体人胰岛胰岛素分泌.结论 iPLA2在人胰岛β细胞高表达并在葡萄糖刺激胰岛β细胞胰岛素分泌过程起到重要的作用.
목적 탐토Ca2+-비의뢰성린산지매A2(iPLA2)재인이도적표체급재이도소분비공능중적작용.방법 정상인이선조직절편면역조직화학염색급인이도Western인적방법검측,관찰iPLA2재인이도중적표체정황;수궤분조대조연구iPLA2선택성억제제추희순내지(BEL)대리체인이도적포도당자격인기이도소분비반응적영향.결과 재인이도iPLA2고표체,여항이도소염색분포일치,이외분비선흔소표체;여대조조상비,BEL처리조이도소분비반응명현감약(P＜0.01),BEL통과억제iPLA2활성억제료포도당자격인기적리체인이도이도소분비.결론 iPLA2재인이도β세포고표체병재포도당자격이도β세포이도소분비과정기도중요적작용.
Objective To assess the role of calcium-independent phospholipase A2 (iPLA2) in human pancreatic islets.Methods The immunohistochemical analysis and Western blot were employed to examine iPLA2 expression in human pancreatic islets. Bromoenol lactone (BEL), a selective inhibitor of iPLA2, was used in a randomized controlled trial to compare its influence to glucose-stimulated insulin secretion.Results iPLA2 was expressed predominantly in islet cells co-stained by insulin but was barely detected in the exocrine acinar cells. Western blot results indicated that islet cells expressed an iPLA2-immunoreactive band at the 80 kDa region. Glucose-stimulated insulin secretory response was dramatically reduced in islets pretreated with BEL (0.8285±0.0803 ng·islet-1·h-1) as compared with the control (1.2264±0.0568 ng·islet-1·h-1) (P＜0.01). BEL inhibited glucose stimulated insulin secretion from isolated human islets.Conclusion iPLA2 signaling plays an important role in glucose-stimulated insulin secretion under the physiological conditions.