CAJ | 학술논문

目的探讨肠上皮干细胞标志分子Msil及Hesl在小鼠急性放射性肠炎(radiation enteritis,RE)模型中的表达情况.方法 40只雌性BALB/c小鼠采用β射线进行以300 cGy/min的速率一次性全腹部照射.选取30只小鼠于照射前(0 d)及照射后第3、5、7、14天每天处死6只,分离小肠,截取小肠中段进行定量PCR、Western印迹及免疫组织化学法测定Msil及Hesl的表达,比较两者的表达差异.结果成功建立小鼠急性RE模型.定量PCR的结果显示,Msil和Hesl在第5天时表达的相对ΔCt值分别为15.17±0.47及15.83±0.24,Western印迹检测结果显示,Msil及Hesl于第5天表达的相对灰度分别为0.443±0.055和0.301±0.047,在RNA及蛋白质水平上两者均显著高于其他时间点(P值均＜0.05).免疫组织化学检测结果显示,Msil和Hesl于第5、7天时在肠隐窝中大量表达.结论 Msil及Hesl在RE的损伤修复前表达上调,提示肠干细胞的增殖与肠上皮修复有关.
목적 탐토장상피간세포표지분자Msil급Hesl재소서급성방사성장염(radiation enteritis,RE)모형중적표체정황.방법 40지자성BALB/c소서채용β사선진행이300 cGy/min적속솔일차성전복부조사.선취30지소서우조사전(0 d)급조사후제3、5、7、14천매천처사6지,분리소장,절취소장중단진행정량PCR、Western인적급면역조직화학법측정Msil급Hesl적표체,비교량자적표체차이.결과 성공건립소서급성RE모형.정량PCR적결과현시,Msil화Hesl재제5천시표체적상대ΔCt치분별위15.17±0.47급15.83±0.24,Western인적검측결과현시,Msil급Hesl우제5천표체적상대회도분별위0.443±0.055화0.301±0.047,재RNA급단백질수평상량자균현저고우기타시간점(P치균＜0.05).면역조직화학검측결과현시,Msil화Hesl우제5、7천시재장은와중대량표체.결론 Msil급Hesl재RE적손상수복전표체상조,제시장간세포적증식여장상피수복유관.
Objective To investigate the expression of intestinal epithelial stem cell markers Msil and Hesl in mouse acute radiation enteritis (RE) model. Methods Forty female BALB/c mice were undergone one-time whole abdominal irradiation by 300 cGy/min β-ray. Thirty mice were selected before irradiation (Day 0) and every six mice were euthanized at day 3,5,7 and 14 after irradiation. The small intestinal were detached and the expression of Msil and Hesl were detected in middle part of small intestinal by quantitative RT-PCR, Western blots, and immunohistochemistry. Subsequently,the difference of Msil and Hesl expression was compared. Results The mouse acute RE model was successfully established. At the 5th day after radiation, the results of quantitative RT-PCR indicated that the relative ΔCt value of Msil and Hesl expression were 15. 17 ± 0. 47 and 15. 83 ± 0. 24respectively, the results of Western blot showed that the integrated intensity were 0. 443±0. 055 and 0. 301 + 0. 047 for Msil and Hesl. The expressions were significantly higher than other time points at RNA and protein levels (P<0. 05). The results of immunohistochemistry revealed that Msil and Hesl were highly expressed in intestinal crypts at 5th day and 7th day. Conclusions The expression of Msil and Hesl, upregulated before wound repair in RE model, indicates that intestinal epithelial repair is related to intestinal stem cell proliferation.