中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINESE JOURNAL OF DIABETES
2004年
1期
52-55
,共4页
烟酰胺%胰腺β细胞%细胞凋亡%一氧化氮
煙酰胺%胰腺β細胞%細胞凋亡%一氧化氮
연선알%이선β세포%세포조망%일양화담
Nitric oxide
目的探讨烟酰胺(NA)对糖尿病(DM)大鼠胰岛β细胞的保护作用. 方法 DM组30只鼠,NA组30只鼠,对照组25只鼠.以NA(1 g/kg)给大鼠灌胃3 d,用链脲佐菌素(STZ)(50 mg/kg)一次性腹腔注射,观察血清一氧化氮(NO)、丙二醛(MDA)、胰腺匀浆一氧化氮合酶(NOS)、超氧化物岐化酶(SOD)及胰岛β细胞凋亡的形态学改变. 结果 1 d时NA组NO为(69±6)μmol/L,DM组为(107±6)μmol/L;5 d时NA组血清MDA为(5.9±1.2)nmol/L,DM组为(9.5±1.0) nmol/L;5 d时NA组胰腺匀浆SOD为(147±13)nU/mg(pro),DM组为(111±6) nU/mg(pro),差异有显著意义(P<0.05);胰腺匀浆NOS三组差异无显著意义(P>0.05).未见有胰岛β细胞过度凋亡. 结论 NA可以清除STZ产生的NO及氧自由基,保护胰岛β细胞,使其不发生过度凋亡对STZ诱导的大鼠糖尿病有预防作用.
目的探討煙酰胺(NA)對糖尿病(DM)大鼠胰島β細胞的保護作用. 方法 DM組30隻鼠,NA組30隻鼠,對照組25隻鼠.以NA(1 g/kg)給大鼠灌胃3 d,用鏈脲佐菌素(STZ)(50 mg/kg)一次性腹腔註射,觀察血清一氧化氮(NO)、丙二醛(MDA)、胰腺勻漿一氧化氮閤酶(NOS)、超氧化物岐化酶(SOD)及胰島β細胞凋亡的形態學改變. 結果 1 d時NA組NO為(69±6)μmol/L,DM組為(107±6)μmol/L;5 d時NA組血清MDA為(5.9±1.2)nmol/L,DM組為(9.5±1.0) nmol/L;5 d時NA組胰腺勻漿SOD為(147±13)nU/mg(pro),DM組為(111±6) nU/mg(pro),差異有顯著意義(P<0.05);胰腺勻漿NOS三組差異無顯著意義(P>0.05).未見有胰島β細胞過度凋亡. 結論 NA可以清除STZ產生的NO及氧自由基,保護胰島β細胞,使其不髮生過度凋亡對STZ誘導的大鼠糖尿病有預防作用.
목적탐토연선알(NA)대당뇨병(DM)대서이도β세포적보호작용. 방법 DM조30지서,NA조30지서,대조조25지서.이NA(1 g/kg)급대서관위3 d,용련뇨좌균소(STZ)(50 mg/kg)일차성복강주사,관찰혈청일양화담(NO)、병이철(MDA)、이선균장일양화담합매(NOS)、초양화물기화매(SOD)급이도β세포조망적형태학개변. 결과 1 d시NA조NO위(69±6)μmol/L,DM조위(107±6)μmol/L;5 d시NA조혈청MDA위(5.9±1.2)nmol/L,DM조위(9.5±1.0) nmol/L;5 d시NA조이선균장SOD위(147±13)nU/mg(pro),DM조위(111±6) nU/mg(pro),차이유현저의의(P<0.05);이선균장NOS삼조차이무현저의의(P>0.05).미견유이도β세포과도조망. 결론 NA가이청제STZ산생적NO급양자유기,보호이도β세포,사기불발생과도조망대STZ유도적대서당뇨병유예방작용.
ObjectiveTo clarify the protective effect of n ic tinamide on beta-cell of islet Langerhands in rats.Methods1 15 rats were divided into control (A group), diabetes (B group) and NA (C group) . NA group rats ingested NA every day. After three days, 2% streptozotocin was i njected intraperitoneum once. Tests were madefor nitric oxide (NO), matondialdeh yde (MDA) in serum, and nitric oxide synthase (NOS), superoxide dismutase (SOD) in pancreas. Apoptotic morphology of Langerhands was checked.Results On day 1, the level of NO was 69.0±5.7 μmol/L in NA group, 106.5±6.57 μmol/L in diabetes group. On day 5, MDA was 5.88±1.21 nmol/L in NA group, 9.5 1±0.97 nmol/L in diabetes group. On day 5, SOD was 146.9±13.1 nU/mg(pro) in NA group, 111.3±6 0 nU/mg(pro) in diabetes group. The difference was significant (P<0.05). There was no difference in NOS among three groups (P>0.05). A nd there were morphological evidences for apoptosis of beta-cells in B group. The apoptosis of beta-cells in C and A groups were not founded.Conclu sionNA acting as a scavenger may protect beta-cells of Langerhands fr om apoptosis. NA may protect rats from sufferring diabetes induced by streptozot ocin.
