CAJ | 학술논문

目的:探讨端粒酶(hTERT)启动子驱动下肿瘤坏死因子相关凋亡诱导配体(TNF-related Apoptosis-inducing Ligand,TRAIL)基因在大肠癌细胞DLDl和HT-29的表达及其杀细胞作用.方法:通过腺病毒载体系统将hTERT启动子驱动的TRAIL基因转入大肠癌细胞DLDl和HT-29,流式细胞仪检测GFP/TRAIL(绿色荧光蛋白和TRAIL融合基因)的表达和细胞凋亡率,MTT法检测细胞生长抑制率.结果:端粒酶启动子驱动的GFP/TRAIL基因和CMV启动子驱动的GFP(绿色荧光蛋白)基因在DLDl和HT-29内的表达率分别达41.63%和31.4%;GFP/TRAIL基因对DLDl和HT-29细胞的生长抑制率分别达93.5%和74.2%,凋亡率分别是41.1%和25.8%,与PBS和Ad/CMV-GFP的差异都有显著意义(P＜0.05).结论:端粒酶启动子驱动的GFP/TRAIL融合基因在大肠癌细胞DLDl和HT-29中能够有效的表达;TRAIL基因对大肠癌细胞DLDl和HT-29有明显的抑制生长和促凋亡作用.
목적:탐토단립매(hTERT)계동자구동하종류배사인자상관조망유도배체(TNF-related Apoptosis-inducing Ligand,TRAIL)기인재대장암세포DLDl화HT-29적표체급기살세포작용.방법:통과선병독재체계통장hTERT계동자구동적TRAIL기인전입대장암세포DLDl화HT-29,류식세포의검측GFP/TRAIL(록색형광단백화TRAIL융합기인)적표체화세포조망솔,MTT법검측세포생장억제솔.결과:단립매계동자구동적GFP/TRAIL기인화CMV계동자구동적GFP(록색형광단백)기인재DLDl화HT-29내적표체솔분별체41.63%화31.4%;GFP/TRAIL기인대DLDl화HT-29세포적생장억제솔분별체93.5%화74.2%,조망솔분별시41.1%화25.8%,여PBS화Ad/CMV-GFP적차이도유현저의의(P＜0.05).결론:단립매계동자구동적GFP/TRAIL융합기인재대장암세포DLDl화HT-29중능구유효적표체;TRAIL기인대대장암세포DLDl화HT-29유명현적억제생장화촉조망작용.