CAJ | 학술논문

[目的]探究龙吐珠最佳组织培养方案.[方法]以马鞭草科龙吐珠叶片、茎段、腋芽为外植体,进行组培快繁技术研究.[结果]最适宜外植体为茎段;最适宜消毒处理是消毒剂为0.1%升汞溶液,消毒时间为6 min.产生侧芽的最佳培养基为1/2 MS+6-BA 1.00 mg/L+NAA 0.05 mg/L+IBA 0.50 mg/L,最适愈伤组织诱导及继代培养基为1/2 MS + 6-BA 2.00 mg/L+NAA 0.10 mg/L,平均诱导率为86.2%;最适分化培养基是1/2 MS +6-BA 1.00 mg/L+ NAA 0.10 mg/L+ KT 0.20 mg/L,平均分化率为76.7%;不定芽的最适生根培养基是1/2 MS+NAA 0.01 mg/L,其生根率为100%.[结论]该方案可为龙吐珠规模化生产提供技术支持.
[목적]탐구룡토주최가조직배양방안.[방법]이마편초과룡토주협편、경단、액아위외식체,진행조배쾌번기술연구.[결과]최괄의외식체위경단;최괄의소독처리시소독제위0.1%승홍용액,소독시간위6 min.산생측아적최가배양기위1/2 MS+6-BA 1.00 mg/L+NAA 0.05 mg/L+IBA 0.50 mg/L,최괄유상조직유도급계대배양기위1/2 MS + 6-BA 2.00 mg/L+NAA 0.10 mg/L,평균유도솔위86.2%;최괄분화배양기시1/2 MS +6-BA 1.00 mg/L+ NAA 0.10 mg/L+ KT 0.20 mg/L,평균분화솔위76.7%;불정아적최괄생근배양기시1/2 MS+NAA 0.01 mg/L,기생근솔위100%.[결론]해방안가위룡토주규모화생산제공기술지지.
[Objective]This research aimed to find out the best tissue culture program of Clerodendrum thomsonae Balf. [Method]With the leaves, stem segments, axillary buds of C.thomsonae as explants, the tissue culture and rapid propagation techniques were studied. [Result]The optimum disinfection method was 0.1% solution of mercuric chloride as the disinfectant, and the disinfection course lasted 6 minutes. The best nutrient medium to produce lateral bud was 1/2 MS+1.00 mg/L 6-BA+0.05 mg/L NAA+0.50 mg/L IBA. The optimum cultivating medium of inducement callus and successive transfer culture was to improve 1/2 MS+2.00 mg/L 6-BA+0.10 mg/L NAA, and its average rate of inducement was 86.2%. The optimum culture medium of differentiation for calli was 1/2 MS +1.00 mg/L 6-BA+0.10 mg/L NAA+0.20 mg/L KT, and its average rate of differentiation was 76.7%. The optimum culture medium of rooting was 1/2 MS+0.01 mg/L NAA, and its average rate of rooting was 100%.[Conclusion]This program can provide technical support for the scale production of Clerodendrum thomsonae Balf.