CAJ | 학술논문

采用0、1.5、3、6 nmol/L紫杉醇处理A549细胞,建立低浓度紫杉醇诱导非小细胞肺癌凋亡模型,分别采用流式细胞术、细胞计数和免疫印迹法分析细胞凋亡、细胞增殖和Bc1-2家族凋亡相关分子的表达.结果表明,低浓度紫杉醇以浓度和时间依赖的方式诱导细胞凋亡并上调Bim分子表达,而对Bc1-2家族其他凋亡相关分子无显著影响.
채용0、1.5、3、6 nmol/L자삼순처리A549세포,건립저농도자삼순유도비소세포폐암조망모형,분별채용류식세포술、세포계수화면역인적법분석세포조망、세포증식화Bc1-2가족조망상관분자적표체.결과표명,저농도자삼순이농도화시간의뢰적방식유도세포조망병상조Bim분자표체,이대Bc1-2가족기타조망상관분자무현저영향.
A549 cells were treated by 0,1.5,3,6 nmol/L paclitaxel to establish non-small cell lung cancer apoptosis model,the apoptosis,cell proliferation and expression of apoptosis-related molecules in Bc1-2 family were analyzed with flow cytometry,cell counting and western blot methods.The results showed that paclitaxel with low concn.induced apoptosis in time and concentration-dependent manner,and up-regulated expression of Bim at the same time,but it had no significant effect on other apoptosis-related molecules in Bc1-2 family.