CAJ | 학술논문

目的探讨卵泡液NO、VEGF、ET-1水平对IVF-ET妊娠结局的预测价值.方法对131例接受IVF-ET的不孕症患者.采用比色法测定其卵泡液内NO水平、ELISA检测其卵泡液内VEGF、ET-1水平.应用经阴道超声于控制性超排卵(COH)周期人绒毛膜促性腺激素(HCG)注射日进行卵巢体积、窦状卵泡数检测.结果 131例患者中临床妊娠49例,妊娠率为37.40%.妊娠组卵泡液内NO、VEGF水平均高于未妊娠组,ET-1水平则低于未妊娠组(P<0.05).HCG注射日,妊娠组总卵巢体积、窦状卵泡数均大于未妊娠组(P<0.05).卵泡液内NO、VEGF水平与总卵巢体积、窦状卵泡数呈正相关.而卵泡液内ET-1水平与总卵巢体积、窦状卵泡数则呈负相关.结论卵泡液内NO、VEGF水平增高.ET-1水平降低,可能促进卵巢局部血流丰富,提高卵巢反应性,利于IVF-ET中妊娠成功.
목적 탐토란포액NO、VEGF、ET-1수평대IVF-ET임신결국적예측개치.방법 대131례접수IVF-ET적불잉증환자.채용비색법측정기란포액내NO수평、ELISA검측기란포액내VEGF、ET-1수평.응용경음도초성우공제성초배란(COH)주기인융모막촉성선격소(HCG)주사일진행란소체적、두상란포수검측.결과 131례환자중림상임신49례,임신솔위37.40%.임신조란포액내NO、VEGF수평균고우미임신조,ET-1수평칙저우미임신조(P<0.05).HCG주사일,임신조총란소체적、두상란포수균대우미임신조(P<0.05).란포액내NO、VEGF수평여총란소체적、두상란포수정정상관.이란포액내ET-1수평여총란소체적、두상란포수칙정부상관.결론 란포액내NO、VEGF수평증고.ET-1수평강저,가능촉진란소국부혈류봉부,제고란소반응성,리우IVF-ET중임신성공.
Objective To investigate the level of follicular fluid NO, VEGF and ET-1 in assessing IVF-ET outcome. Methods Totally 131 patients undergoing IVF-ET cycles were recruited. The level of follicular fluid NO was measured by chromatometry. The follicular fluid VEGF and ET-1 were measured by ELISA. Transvaginal ultrasound was performed on human chorionic gonadotropin (hCG) injection day to determine ovarian volume and antral follicle count. Results The pregnancy rate was 37.40% (49/131). There were significantly increased level of follicular fluid NO, VEGF and decreased level of follicular fluid ET-1 in the pregnant group than those in the non-pregnant group (P<0.05). Total ovarian volume and antral follicle count on HCG injection day were significantly higher in the pregnant group than those in the non-pregnant group (P<0.05). The levels of follicular fluid NO and VEGF had positive correlations with the total ovarian volume and antral follicle count. However, the level of follicular fluid ET-1 had a negative correlation with the total ovarian volume and antral follicle count. Conclusions The high level of NO, VEGF and low level of ET-1 in follicular fluid are good predictors of ovarian blood flow and ovarian response in IVF-ET.