东南大学学报（医学版）
東南大學學報（醫學版）
동남대학학보（의학판）
JOURNAL OF SOUTHEAST UNIVERSITY(MEDICAL SCIENCE EDITION)
2010年
1期
36-41
,共6页
热激胁迫%脂肪%糖原%发育%涡虫
熱激脅迫%脂肪%糖原%髮育%渦蟲
열격협박%지방%당원%발육%와충
heat-shock%lipid%glycogen%development%planarian
目的:探讨热激胁迫对于涡虫发育以及脂肪和糖原积累的影响.方法:涡虫的发育通过体长与体质量来评价.分析35 ℃热激胁迫处理涡虫的体长与体质量变化以及实质细胞中脂肪与糖原积累情况,并评价热激胁迫处理涡虫中发育与脂肪或糖原积累之间可能的相关性.结果:与未经历热激处理的对照相比,热激胁迫处理不同长度时间(16、20、24、28与32 h)后涡虫体长与体质量显著减少或降低.与对照相比,热激胁迫处理导致涡虫实质细胞中的脂肪颗粒数量明显降低、尺寸显著变小.类似地,热激胁迫处理还导致涡虫实质细胞中的糖原积累的显著降低.进而,线性回归分析表明热激胁迫处理涡虫的体长与体质量均显著相关于实质细胞中脂肪颗粒的尺寸或糖原的含量.结论:热激胁迫处理可以明显抑制涡虫的发育,且热激胁迫处理涡虫中存在发育与脂肪或糖原积累之间的显著关联.
目的:探討熱激脅迫對于渦蟲髮育以及脂肪和糖原積纍的影響.方法:渦蟲的髮育通過體長與體質量來評價.分析35 ℃熱激脅迫處理渦蟲的體長與體質量變化以及實質細胞中脂肪與糖原積纍情況,併評價熱激脅迫處理渦蟲中髮育與脂肪或糖原積纍之間可能的相關性.結果:與未經歷熱激處理的對照相比,熱激脅迫處理不同長度時間(16、20、24、28與32 h)後渦蟲體長與體質量顯著減少或降低.與對照相比,熱激脅迫處理導緻渦蟲實質細胞中的脂肪顆粒數量明顯降低、呎吋顯著變小.類似地,熱激脅迫處理還導緻渦蟲實質細胞中的糖原積纍的顯著降低.進而,線性迴歸分析錶明熱激脅迫處理渦蟲的體長與體質量均顯著相關于實質細胞中脂肪顆粒的呎吋或糖原的含量.結論:熱激脅迫處理可以明顯抑製渦蟲的髮育,且熱激脅迫處理渦蟲中存在髮育與脂肪或糖原積纍之間的顯著關聯.
목적:탐토열격협박대우와충발육이급지방화당원적루적영향.방법:와충적발육통과체장여체질량래평개.분석35 ℃열격협박처리와충적체장여체질량변화이급실질세포중지방여당원적루정황,병평개열격협박처리와충중발육여지방혹당원적루지간가능적상관성.결과:여미경력열격처리적대조상비,열격협박처리불동장도시간(16、20、24、28여32 h)후와충체장여체질량현저감소혹강저.여대조상비,열격협박처리도치와충실질세포중적지방과립수량명현강저、척촌현저변소.유사지,열격협박처리환도치와충실질세포중적당원적루적현저강저.진이,선성회귀분석표명열격협박처리와충적체장여체질량균현저상관우실질세포중지방과립적척촌혹당원적함량.결론:열격협박처리가이명현억제와충적발육,차열격협박처리와충중존재발육여지방혹당원적루지간적현저관련.
Objective: To investigate the effects of heat-shock on development and accumulation of lipid and glycogen in planarian Dugesia japonica. Methods: The development of planarians was evaluated by the body length and body weight. The body length, body weight, and the accumulation of lipid and glycogen in parenchymal cells were examined in heat-shock(35 ℃) treated planarians. The possible associations of development with lipid or glycogen storage were further analyzed in heat-shock treated planarians. Results: After heat-shock treatment for different time intervals(16, 20, 24, 28, and 32h), the body weights and body lengths of heat-shock treated planarians were all significantly decreased compared with those in control planarians without heat-shock treatment. Compared with the lipid accumulation in control planarians, heat-shock treatment resulted in the sharp decrease of lipid droplet number and the reduction of lipid droplet size in parenchymal cells. Similarly, the relative intensities of labeled signals for glycogen in parenchymal cells were significantly decreased after heat-shock treatment. Moreover, linear regression analysis suggests that both the body weight and the body length were significantly correlated with the relative size of lipid droplet and the relative intensity of glycogen signal in heat-shock treated planarians. Conclusion: Treatment with the stress of heat-shock can obviously suppress the development of planarians, and the close association between the development and the accumulation of lipid or glycogen exists in heat-shock treated planarians.
