CAJ | 학술논문

建立了一种基于胶束增敏缔合反应水相测定乳制品中三聚氰胺的分光光度法,研究了在阴离子表面活性剂十二烷基硫酸钠(SDS)形成的胶束体系中,茜素红(AR)阴离子与三聚氰胺阳离子反应产物的紫外-可见光谱特性.实验发现,加入三聚氰胺后,体系λmax由426 nm红移到516 nm,加入SDS对缔合物有显著的增稳、增敏效应.三聚氰胺质量浓度在1.9～25 mg/L范围内符合比尔定律,缔合物的表观摩尔吸光系数为6.9×10~3 L·mol~(-1)·cm~(-1).本方法用于液态奶及奶粉中三聚氰胺的测定,回收率分别为94.5%～104.8%和95.5%～100.4%,相对标准偏差分别为1.6%～2.2%和1.7%～2.6%.考察了酸效应、胶束对体系的作用机理,发现三聚氰胺与AR缔合物之间主要是通过静电引力和疏水作用力相结合.
건립료일충기우효속증민체합반응수상측정유제품중삼취청알적분광광도법,연구료재음리자표면활성제십이완기류산납(SDS)형성적효속체계중,천소홍(AR)음리자여삼취청알양리자반응산물적자외-가견광보특성.실험발현,가입삼취청알후,체계λmax유426 nm홍이도516 nm,가입SDS대체합물유현저적증은、증민효응.삼취청알질량농도재1.9～25 mg/L범위내부합비이정률,체합물적표관마이흡광계수위6.9×10~3 L·mol~(-1)·cm~(-1).본방법용우액태내급내분중삼취청알적측정,회수솔분별위94.5%～104.8%화95.5%～100.4%,상대표준편차분별위1.6%～2.2%화1.7%～2.6%.고찰료산효응、효속대체계적작용궤리,발현삼취청알여AR체합물지간주요시통과정전인력화소수작용력상결합.
A novel spectrophotometric method for the determination of melamine has been developed based on the association reaction with micelle-stabilized in aqueous phase. The ultraviolet-visible spectral characteristics of the association complex formed between melamine and alizarin red in sodium dodecylsulfate (SDS) micellar system were studied. Experimental results showed the maximum absorption of alizarin red was shifted from 426 nm to 516 nm when the complex were formed, that stability and sensitivity of the complex was remarkably enhanced by SDS micellar system. Beer's law was obeyed in the concentration range of 1.9～25 mg/L. The apparent molar absorptivity of the complex was 6.9×10~3 L·mol~(-1)·cm~(-1) at 516 nm. The proposed method had been applied to the determination of melamine in liquid milk and milk powder. The recoveries were 94.5%～104.8% and 95.5%～100.4%. The relative standard deviations were between 1.6%～2.2% and 1.7%～2.6%. The association complex is formed chiefly by electrostatic attractions and hydrophobic interactions due to acidic effect and micella.