食品科学
食品科學
식품과학
FOOD SCIENCE
2010年
4期
236-238
,共3页
反相高效液相色谱法%秤锤树%熊果酸
反相高效液相色譜法%秤錘樹%熊果痠
반상고효액상색보법%칭추수%웅과산
HPLC%Sinojackia sarcocarpa%ursolic acid
目的:建立肉果秤锤树树叶中熊果酸含量的高效液相色谱测定方法.方法:采用Shim-pack VP-ODS C_(18)柱(150mm×4.6mm,5μm),甲醇-水-磷酸(850:147:3,V/Y)为流动相,检测波长210nm,流速1.0mL/min.结果:熊果酸的进样最在0.124～2.48 μg/μL范围内与峰面秘积分值具有良好的线性关系(R~2=0.9971),平均回收率为100.5%,RSD为0.91%.超声提取的熊果酸含量最高.结论:该方法稳定性、重现性好,且简单易行.
目的:建立肉果秤錘樹樹葉中熊果痠含量的高效液相色譜測定方法.方法:採用Shim-pack VP-ODS C_(18)柱(150mm×4.6mm,5μm),甲醇-水-燐痠(850:147:3,V/Y)為流動相,檢測波長210nm,流速1.0mL/min.結果:熊果痠的進樣最在0.124～2.48 μg/μL範圍內與峰麵祕積分值具有良好的線性關繫(R~2=0.9971),平均迴收率為100.5%,RSD為0.91%.超聲提取的熊果痠含量最高.結論:該方法穩定性、重現性好,且簡單易行.
목적:건립육과칭추수수협중웅과산함량적고효액상색보측정방법.방법:채용Shim-pack VP-ODS C_(18)주(150mm×4.6mm,5μm),갑순-수-린산(850:147:3,V/Y)위류동상,검측파장210nm,류속1.0mL/min.결과:웅과산적진양최재0.124～2.48 μg/μL범위내여봉면비적분치구유량호적선성관계(R~2=0.9971),평균회수솔위100.5%,RSD위0.91%.초성제취적웅과산함량최고.결론:해방법은정성、중현성호,차간단역행.
A HPLC method was developed for the determination of ursolic acid in leaves of Sinojackia sarcocarpa L.Q.Luo.The chromatographic separation was achieved on a Shim-pack VP-ODS C_(18) column using a mobile phase composed of H_2O and H3PO4(850:147:3,V/V)at a flow rate of 1 mL/min,and the detection wavelength was set at 210 nm.A good linearity of the standard curve for peak area versus ursolic acid concentration was obtained over the range of 0.124-2.48 μg/μL(Re = 0.9971).The average spike recovery of ursolic acid at three levels was 100.5%(n = 3),with 0.91% RSD.In sample preparation,ultrasonic extraction resulted in higher efficiency when compared to reflux extraction and percolation extraction.This method reveals the characteristics of good stability and repeatability,simplicity,and easy operation.
