中国医药工业杂志
中國醫藥工業雜誌
중국의약공업잡지
CHINESE JOURNAL OF PHARMACEUTICALS
2010年
1期
41-43
,共3页
夏怡然%王健%朱金屏%陈芳%李丁
夏怡然%王健%硃金屏%陳芳%李丁
하이연%왕건%주금병%진방%리정
毛萼乙素%高效液相色谱%测定
毛萼乙素%高效液相色譜%測定
모악을소%고효액상색보%측정
eriocalyxin B%HPLC%determination
建立了HPLC法测定大鼠血浆中的毛萼乙素.采用液相萃取法处理血浆样品,色谱柱为C_(18)柱,流动相为乙腈-水(43:57),检测波长233nm.毛萼乙素在40～8 000 ng/ml浓度范围内线性关系良好.方法回收率101.6%～106.5%,日内、日间RSD为4.3%～12.4%.
建立瞭HPLC法測定大鼠血漿中的毛萼乙素.採用液相萃取法處理血漿樣品,色譜柱為C_(18)柱,流動相為乙腈-水(43:57),檢測波長233nm.毛萼乙素在40～8 000 ng/ml濃度範圍內線性關繫良好.方法迴收率101.6%～106.5%,日內、日間RSD為4.3%～12.4%.
건립료HPLC법측정대서혈장중적모악을소.채용액상췌취법처리혈장양품,색보주위C_(18)주,류동상위을정-수(43:57),검측파장233nm.모악을소재40～8 000 ng/ml농도범위내선성관계량호.방법회수솔101.6%～106.5%,일내、일간RSD위4.3%～12.4%.
An HPLC method was established for the determination of eriocalyxin B in rat plasma.Eriocalyxin B was extracted by liquid-liquid extraction.A C_(18) column was used with the mobile phase of acetonitrile-water(43:57)at the detection wavelength of 233 nm.The calibration curvg was linear in the concentration range of 40-8 000 ng/m1.The recoveries were 101.6%-106.5% with intra-and inter-day RSDs of4.3%-12.4%.
